Manipulating specific nutrients in growth media to boost production of lipids or carotenoids in microalgae is a widespread practice; however, it decreases overall biomass productivity, an important consideration for viable production of biofuels. In the present study, maximum productivity of lipids (117.01 mg/L/d) and biomass (249.60 mg/L/d) was obtained on day 13 in Bold's basal medium (BBM) modified by adding 25 mM NaCl and 20 μM phosphate. Lutein and astaxanthin productivity was, however, only slightly different from that in the standard medium, namely blue-green-11 (BG-11). The lipids profile was dominated by C16:0 and C 18:1 fatty acids ranging from 26.69 to 39.32% and 10.8–35.31% (palmitic and oleic acids) in all the five variants of the culture medium. These results indicate that the highest proportion of saturated fatty acids (SFA; 61.02%) was obtained in BG-11 whereas that of monounsaturated fatty acids (MUFA; 37.34%) was obtained in BBM supplemented with NaCl (50 mM) and phosphate (20 μM). All the five variants led to lipids suitable for biodiesel production, marked by a lipid profile dominated by the two fatty acids. It is concluded that making the culture medium more saline and enriching it with phosphates increased lipid and biomass productivity and the synthesis of fatty acids methyl esters (FAME).

在生长培养基中处理特定营养素以提高微藻类中脂质或类胡萝卜素的产量是一种普遍的做法。但是，它降低了整体生物质生产率，这是可行生产生物燃料的重要考虑因素。在本研究中，在第13天，在通过添加25 mM NaCl和20μM磷酸盐改性的Bold基础培养基（BBM）中获得了脂质（117.01 mg / L / d）和生物质（249.60 mg / L / d）的最大生产力。但是，叶黄素和虾青素的生产率与标准培养基（蓝绿色11（BG-11））相比仅稍有不同。在所有五个变体中，C16：0和C 18：1脂肪酸占26.69％至39.32％和10.8-35.31％（棕榈酸和油酸）的脂类谱。这些结果表明饱和脂肪酸的比例最高（SFA; 61。在BG-11中获得了02％），而在补充有NaCl（50 mM）和磷酸盐（20μM）的BBM中获得了单不饱和脂肪酸（MUFA; 37.34％）。所有这五个变体导致产生适合生物柴油生产的脂质，其特征在于由两种脂肪酸主导的脂质谱。结论是，增加培养基的盐分并使其富含磷酸盐会增加脂质和生物质的生产率以及脂肪酸甲酯的合成（FAME）。