Yam (.), as an important traditional medicinal herb, has a functional impact upon anti-disease and strengthens immunity. In the present study, we conducted growth performance, serum biochemical level, metabonomics analysis of liver in rainbow trout () fed diets including yam extract (0, 0.2 %, and 0.4 %) for 56 days, to identify yam extract-affected growth performance, serum biochemical level, liver metabolites and metabolic pathways. After the feeding experiment, there were no significant changes in weight gain rate (WGR) and specific growth rate (SGR) in yam-supplemented groups. Albumin (ALB) and total protein (TP) contents, and superoxide dismutase (SOD) and acid phosphatase (ACP) activities were increased, while alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities were decreased in yam extract groups. Using VIP- values > 1 and -value < 0.05 as the threshold, a total of 29 and 78 metabolites were significantly up-regulated in 0.2 % and 0.4 % yam extract group, respectively, while 14 and 74 metabolites were significantly down-regulated. Moreover, 18 overlap significantly discriminating metabolites in two comparisons were annotated to 8 metabolic pathways in KEGG analysis, 7 top significantly enriched pathways terms ( < 0.05) include Glycosylphosphatidylinositol (GPI)-anchor biosynthesis, Autophagy-other, Autophagy-animal, Ferroptosis, Glutathione metabolism, Glycerophospholipid metabolism, and Cysteine and methionine metabolism. Results showed that yam extract has certain antioxidant and protective effects on liver of rainbow trout, and the mechanism of this effect may be related to the conversion of Glycerophosphoethanolamine to glycerophosphocholine, down-regulation of canavanine, glutathione metabolism and Cysteine and methionine metabolism pathways.

中文翻译：

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山药 (Dioscorea oppositifolia L.) 对虹鳟 (Oncorhynchus mykiss) 生长性能、血清生化水平和肝脏代谢组学的影响


山药作为重要的传统药材，具有抗病、增强免疫力等功能。在本研究中，我们对饲喂含有山药提取物（0、0.2% 和 0.4%）的虹鳟鱼肝脏进行了 56 天的生长性能、血清生化水平和代谢组学分析，以确定山药提取物对生长性能的影响、血清生化水平、肝脏代谢物和代谢途径。饲喂实验后，山药补充组的增重率（WGR）和特定生长率（SGR）没有显着变化。山药提取物组的白蛋白（ALB）和总蛋白（TP）含量以及超氧化物歧化酶（SOD）和酸性磷酸酶（ACP）活性增加，而丙氨酸转氨酶（ALT）和碱性磷酸酶（ALP）活性降低。以VIP-值> 1和-值< 0.05为阈值，0.2%和0.4%山药提取物组中分别有29和78种代谢物显着上调，而14和74种代谢物显着下调。此外，两次比较中18个重叠的显着区分代谢物在KEGG分析中被注释为8个代谢途径，7个显着富集的最高途径术语（<0.05）包括糖基磷脂酰肌醇（GPI）-锚定生物合成、自噬-其他、自噬-动物、铁死亡、谷胱甘肽代谢、甘油磷脂代谢、半胱氨酸和蛋氨酸代谢。 结果表明，山药提取物对虹鳟鱼的肝脏具有一定的抗氧化和保护作用，其作用机制可能与甘油磷酸乙醇胺转化为甘油磷酸胆碱，下调刀豆氨酸、谷胱甘肽代谢以及半胱氨酸和蛋氨酸代谢途径有关。