Abstract A label-free electrochemical biosensor with magnetically induced self-assembly based on peptide nucleic acids (PNA) and Fe3O4/Fe2O3 heteroplasmon nanorods was successfully fabricated to detect the single nucleotide polymorphism (SNP) of CYP2C9*3. The sensitivity of the electrochemical biosensor was improved by introducing PNA and Fe3O4/Fe2O3@Au nanocomposites. The Fe3O4/Fe2O3@Au nanocomposites were assembled on the surface of the magnetic glassy carbon electrode (MGCE) by magnetic field, and the thiolated PNA were connected to the nanocomposites through Au-S bonds. The biosensor exhibited a good linear correlation between 1 pM and 1 µM (R2 = 0.9941), and the limit of detection (LOD) and the limit of quantitation (LOQ) were 0.95 pM and 3.18 pM, respectively. Besides, the electrochemical biosensor could recognize single-base mismatch DNA, and it could be stored stably for 15 days at 4 °C.

中文翻译：

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一种用于检测CYP2C9*3基因的磁诱导自组装无标记电化学生物传感器

摘要 成功制备了一种基于肽核酸（PNA）和Fe3O4/Fe2O3异质子纳米棒磁诱导自组装的无标记电化学生物传感器，用于检测CYP2C9*3的单核苷酸多态性（SNP）。通过引入 PNA 和 Fe3O4/Fe2O3@Au 纳米复合材料，提高了电化学生物传感器的灵敏度。Fe3O4/Fe2O3@Au纳米复合材料通过磁场组装在磁性玻碳电极（MGCE）的表面，硫醇化的PNA通过Au-S键连接到纳米复合材料上。该生物传感器在 1 pM 和 1 µM 之间表现出良好的线性相关性 (R2 = 0.9941)，检测限 (LOD) 和定量限 (LOQ) 分别为 0.95 pM 和 3.18 pM。此外，电化学生物传感器可以识别单碱基错配 DNA，