当前位置: X-MOL 学术Planta › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Overexpression of wheat transcription factor (TaHsfA6b) provides thermotolerance in barley
Planta ( IF 3.6 ) Pub Date : 2020-09-18 , DOI: 10.1007/s00425-020-03457-4
Anuj Kumar Poonia 1 , Sumit Kumar Mishra 1 , Parul Sirohi 1, 2 , Reeku Chaudhary 1 , Meenakshi Kanwar 1 , Hugo Germain 2 , Harsh Chauhan 1
Affiliation  

MAIN CONCLUSION Overexpressing a heat shock factor gene (TaHsfA6bT) from wheat provides thermotolerance in barley by constitutive expression of heat and other abiotic stress-response genes. Temperature is one of the most crucial abiotic factors defining the yield potential of temperate cereal crops, such as barley. The regulators of heat shock response (HSR), heat stress transcription factors (Hsfs), modulate the transcription level of heat-responsive genes to protect the plants from heat stress. In this study, an Hsf from wheat (TaHsfA6b) is overexpressed in barley for providing thermotolerance. Transgenic barley lines overexpressing TaHsfA6b showed improvement in thermotolerance. The constitutive overexpression of a TaHsfA6b gene upregulated the expression of major heat shock proteins and other abiotic stress-responsive genes. RNA-seq and qRT-PCR analysis confirmed the upregulation of Hsps, chaperonins, DNAJ, LEA protein genes, and genes related to anti-oxidative enzymes in transgenic lines. Excessive generation and accumulation of reactive oxygen species (ROS) occurred in wild-type (WT) plants during heat stress; however, the transgenic lines reflected improved ROS homeostasis mechanisms, showing lesser ROS accumulation under high temperature. No negative phenotypic changes were observed in overexpression lines. These results suggest that TaHsfA6b is a regulator of HSR and its overexpression altered the expression patterns of some main stress-related genes and enhanced the thermotolerance of this cereal crop.

中文翻译:

小麦转录因子 (TaHsfA6b) 的过表达提供了大麦的耐热性

主要结论 从小麦中过表达热激因子基因 (TaHsfA6bT) 通过热和其他非生物胁迫响应基因的组成型表达在大麦中提供耐热性。温度是决定大麦等温带谷类作物产量潜力的最关键的非生物因素之一。热休克反应(HSR)的调节因子,热应激转录因子(Hsfs),调节热反应基​​因的转录水平,以保护植物免受热应激。在这项研究中,来自小麦的 Hsf (TaHsfA6b) 在大麦中过表达以提供耐热性。过表达 TaHsfA6b 的转基因大麦系显示出耐热性的改善。TaHsfA6b 基因的组成型过表达上调了主要热休克蛋白和其他非生物应激反应基因的表达。RNA-seq 和 qRT-PCR 分析证实了转基因株系中 Hsps、伴侣蛋白、DNAJ、LEA 蛋白基因和抗氧化酶相关基因的上调。野生型 (WT) 植物在热应激期间会产生过量的活性氧 (ROS) 和积累;然而,转基因品系反映了改善的 ROS 稳态机制,在高温下显示出较少的 ROS 积累。在过表达系中未观察到负表型变化。这些结果表明,TaHsfA6b 是 HSR 的调节因子,其过表达改变了一些主要的胁迫相关基因的表达模式,增强了这种谷类作物的耐热性。野生型 (WT) 植物在热应激期间会产生过量的活性氧 (ROS) 和积累;然而,转基因品系反映了改善的 ROS 稳态机制,在高温下显示出较少的 ROS 积累。在过表达系中未观察到负表型变化。这些结果表明,TaHsfA6b 是 HSR 的调节因子,其过表达改变了一些主要的胁迫相关基因的表达模式,增强了这种谷类作物的耐热性。野生型 (WT) 植物在热应激期间会产生过量的活性氧 (ROS) 和积累;然而,转基因品系反映了改善的 ROS 稳态机制,在高温下显示出较少的 ROS 积累。在过表达系中未观察到负表型变化。这些结果表明,TaHsfA6b 是 HSR 的调节因子,其过表达改变了一些主要的胁迫相关基因的表达模式,增强了这种谷类作物的耐热性。
更新日期:2020-09-18
down
wechat
bug