Recent molecular studies suggest that Cryptococcus may inhabit the normal human mouth. We attempted to isolate Cryptococcus from 21 adult non-acutely ill patients and 40 volunteer medical and non-medical staff in Southeastern Wisconsin, USA. An upper lip sulcus culture and an oral rinse specimen were inoculated separately onto Staib (birdseed) agar containing chloramphenicol and incubated in gas impermeable zip lock bags at 35 °C. No cryptococci were grown from any of the 122 samples from the 61 subjects. Both specimens from a woman with no risk factors for fungal disease yielded a black yeast at 4 days on Staib agar. This isolate was shown to be Exophiala dermatitidis by colony and microscopic morphology, analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and sequencing through the internal transcribed spacer ribosomal RNA gene. This appears to be a novel isolation of E. dermatitidis from the oral cavity of a generally healthy human.

最近的分子研究表明，隐球菌可能存在于正常人的口腔中。我们试图从美国威斯康星州东南部的 21 名成年非急性患者和 40 名志愿医疗和非医疗人员中分离出隐球菌。将上唇沟培养物和口腔冲洗液样本分别接种到含有氯霉素的 Staib（鸟籽）琼脂上，并在不透气的自封袋中于 35°C 下孵育。来自 61 名受试者的 122 份样本中的任何一份均未生长出隐球菌。来自一名没有真菌病危险因素的女性的两个标本在 Staib 琼脂上培养 4 天后都产生了黑色酵母。该分离株被证明是Exophiala dermatitidis通过菌落和微观形态、基质辅助激光解吸/电离飞行时间质谱分析以及通过内部转录间隔核糖体 RNA 基因测序。这似乎是从一般健康人的口腔中分离出皮炎大肠杆菌的新方法。