The study of senescence preservative on cut flowers helps boost the commercial value of flowers. Senescence in cut flower is associated with an increase of ethylene production, and is significantly influenced by ethylene pathway. This study was conducted to investigate whether S-adenosyl-L-methionine (SAM) and aminocyclopropane-1-carboxylic acid (ACC) involved in the ethylene synthesis process are correlated with the lysosome. The alterations of lysosome which was treated with the ethylene precursors ACC and SAM in HeLa cell using the confocal laser scanning microscope were investigated. According to the experimental results, the activity of lysosomes increased concentration dependently by ACC treatment, however, no change was observed by SAM treatment. In addition, Liquid chromatography–mass spectrometry (LC/MS) analysis was performed to confirm the effect of lysosomal enzyme (LE) extracted from egg white on ACC reduction, but no change was observed. On the contrary, to confirm the effect of ACC on lysosomes, lysosomes were extracted from HeLa cells treated with 5 mM ACC and confirmed by FE-SEM. The results showed that the size of lysosomes treated with ACC is larger than that of the control, which was treated with distilled water. The lysosomes in the control group were distributed in various ranges from 0 to 800 nm, but those treated with 5 mM ACC were in the range of 400 nm to 800 nm or more. Therefore, lysosomes had no effect on ACC, the precursor of ethylene, the aging hormone of cut flowers, however, ACC had effect on lysosomes.

对切​​花的衰老防腐剂的研究有助于提高花的商业价值。切花的衰老与乙烯产量的增加有关，并且受到乙烯途径的显着影响。进行这项研究以调查参与乙烯合成过程的S-腺苷-L-甲硫氨酸（SAM）和氨基环丙烷-1-羧酸（ACC）是否与溶酶体相关。利用共聚焦激光扫描显微镜研究了乙烯前体ACC和SAM处理HeLa细胞中溶酶体的变化。根据实验结果，溶酶体的活性通过ACC处理依赖于浓度增加，但是，通过SAM处理未观察到变化。此外，进行液相色谱-质谱（LC / MS）分析以确认从蛋清中提取的溶酶体酶（LE）对ACC减少的影响，但未观察到变化。相反，为了确认ACC对溶酶体的作用，从用5mM ACC处理的HeLa细胞中提取溶酶体并通过FE-SEM进行确认。结果显示，用ACC处理的溶酶体的大小大于用蒸馏水处理的对照的溶酶体的大小。对照组中的溶酶体分布在0至800nm的各个范围内，但是用5mM ACC处理的溶酶体在400nm至800nm或更大的范围内。因此，溶酶体对ACC，乙烯的前体，切花的衰老激素没有影响，而ACC对溶酶体有影响。