Chlortetracycline (CTC) has been widely used in veterinary medicine in recent years, which has resulted in severe environmental issues due to its low degradation rate and high risk to induce antibiotic resistance bacteria and genes. In previous studies, CTC could be efficiently degraded by Trichoderma harzianum LJ245. Nevertheless, the strain itself suffers from relatively poor adaptability due to the limited number of spores produced. In this paper, ultraviolet (UV) mutagenesis was conducted on LJ245, and various mutants with high sporulation rate were generated to expand the environmental adaptability and enhance CTC degradation. An OmniLog-based method, where 95 types of carbon sources were applied, was first proposed to acquire the carbon metabolic profile of the strains. Several controlled experiments were performed to evaluate the impact of co-substrate metabolism on strain growth, CTC biodegradation, and metabolites biotoxicity removal. The result shows that produced mutants could significantly broaden the carbon metabolic profile and expand the environmental adaptability compared to the original LJ245, where the mutants obtained remarkable increase in total number of usable carbon sources. Meanwhile, as the sole carbon source, CTC could not be fully degraded by the strains. However, the use of co-metabolism could considerably enhance CTC degradation and completely remove CTC degradation products biotoxicity by all strains. Specifically, amino acids and carboxylic acids had the best performance on both strain growth and CTC degradation among all carbon source categories. The results can be applied to the biodegradation treatment of CTC in solid residue, waste water and other environments.

近年来，金霉素（CTC）已广泛用于兽药中，由于其降解速度慢，诱导抗生素抗性细菌和基因的风险高，导致了严重的环境问题。在以前的研究中，哈茨木霉可以有效降解四氯化碳LJ245。然而，由于产生的孢子数量有限，菌株本身具有相对较差的适应性。本文在LJ245上进行了紫外线诱变，并产生了具有高孢子形成率的各种突变体，以扩大环境适应性并增强CTC降解。首先提出了一种基于OmniLog的方法，其中应用了95种碳源，以获取菌株的碳代谢谱。进行了几个对照实验，以评估共底物代谢对菌株生长，CTC生物降解和代谢物生物毒性去除的影响。结果表明，与原始LJ245相比，产生的突变体可以显着拓宽碳代谢谱并扩展环境适应性，突变体获得的可用碳源总数显着增加。同时，作为唯一的碳源，四氯化碳不能被菌株完全降解。但是，使用共代谢可以显着增强CTC降解并完全消除所有菌株产生的CTC降解产物的生物毒性。具体而言，在所有碳源类别中，氨基酸和羧酸在菌株生长和CTC降解方面均表现最佳。该结果可用于四氯化碳在固体残留物，废水和其他环境中的生物降解处理。使用共代谢可以显着增强四氯化碳的降解，并完全消除所有菌株产生的四氯化碳降解产物的生物毒性。具体而言，在所有碳源类别中，氨基酸和羧酸在菌株生长和CTC降解方面均表现最佳。该结果可用于四氯化碳在固体残留物，废水和其他环境中的生物降解处理。共代谢的使用可以大大增强四氯化碳的降解，并完全消除所有菌株的四氯化碳降解产物的生物毒性。具体而言，在所有碳源类别中，氨基酸和羧酸在菌株生长和CTC降解方面均表现最佳。该结果可用于四氯化碳在固体残留物，废水和其他环境中的生物降解处理。