Purpose

This study was carried out to determine the paramphistome species parasitizing water buffaloes (WBs) grazing in the Kızılırmak Delta in Samsun Province, Turkey, and the intermediate hosts of the parasites.

Methods

Between August 2016 and July 2018, abattoirs in Samsun Province were visited weekly and 139 slaughtered WBs were examined for paramphistome species. In the same period, 550 snails (300 Galba truncatula, 200 Physella (Physa) acuta and 50 Planorbis planorbis) were collected from pastures grazed by WBs during monthly sampling in the spring and autumn. Adult parasites were identified through the use of histological and molecular methods and larval stages were identified with a molecular method.

Results

Forty-five of the 139 WBs (32.4%) were infected with species of the family Paramphistomidae and a total of 4761 (mean 105.8) parasites were collected from them. The genera Paramphistomum and Calicophoron were distinguished from each other by examining the development status of the pars musculosa and the degree of lobulation of the testes in histological sections. Calicophoron daubneyi was present in all the infected animals and Paramphistomum cervi in only 3 of the same animals. There were larval forms in only 19 of the specimens identified as G. truncatula. Calicophoron daubneyi was molecularly diagnosed in 12 of the 19 infected G. truncatula and this result was confirmed by PCR and PCR–RFLP. Calicophoron daubneyi was also identified molecularly as being present in all 45 infected adult WBs and as developmental stages in 12 of 300 (4%) intermediate hosts, G. truncatula. The DNA sequences from the adult parasites in the definitive hosts and larval forms in intermediate hosts were allocated the codes MH939278 and MH939279, respectively, in GenBank.

Conclusion

For the first time in Turkey, C. daubneyi was identified molecularly and its intermediate host was identified as G. truncatula. Calicophoron daubneyi was identified as the overwhelmingly dominant paramphistome species in WBs in the Kızılırmak Delta, with P. cervi found in mixed infections in only three animals.

中文翻译：

---

土耳其萨姆松省 Kızılırmak 三角洲的水牛和中间宿主的 Paramphistome 物种。

目的

进行这项研究是为了确定寄生在土耳其萨姆松省 Kızılırmak 三角洲放牧的水牛 (WBs) 的寄生虫物种，以及寄生虫的中间宿主。

方法

2016 年 8 月至 2018 年 7 月期间，每周访问萨姆松省的屠宰场，并检查了 139 只屠宰的 WBs 中的副吸虫物种。同期，在春秋两季每月取样时，从WBs放牧的牧场上采集了550只蜗牛（300只Galba truncatula、200只Physella (Physa) acuta和50只Planorbis planorbis）。通过使用组织学和分子方法鉴定成虫，并用分子方法鉴定幼虫阶段。

结果

139 个 WB 中有 45 个（32.4%）感染了 Paramphistomidae 科的物种，从它们中收集了总共 4761 个（平均 105.8 个）寄生虫。Paramphistomum属和Calicophoron属通过检查组织切片中的肌部发育状况和睾丸分叶程度来区分。Calicophoron daubneyi存在于所有受感染的动物中，而Paramphistomum cervi 仅存在于 3 只相同的动物中。只有 19 个被鉴定为G. truncatula的标本中存在幼虫形式。Calicophoron daubneyi在 19 个受感染的G. truncatula中的 12 个中被分子诊断出这一结果得到了 PCR 和 PCR-RFLP 的证实。Calicophoron daubneyi在分子上也被鉴定为存在于所有 45 个受感染的成年 WB 中，并且在 300 个（4%）中间宿主中的 12 个G 中处于发育阶段。截形。来自最终宿主中成虫和中间宿主中幼虫形式的 DNA 序列分别在 GenBank 中分配了代码 MH939278 和 MH939279。

结论

C. daubneyi在土耳其首次被分子鉴定，其中间宿主被鉴定为G. truncatula。Calicophoron daubneyi被确定为 Kızılırmak Delta 中 WBs 中压倒性优势的 paramphistome 物种，仅在三只动物的混合感染中发现了P. cervi。