Botroclot is a marketed preparation containing hemocoagulase, which is an enzyme having coagulant activity, isolated from the snake Botrops atrox. This formulation is used in dental surgeries and other minor surgical wounds. However, the formulation remains untested in diabetic wounds. Hence, we proposed a study for the topical application of Botroclot in high-fat diet (HFD) + Streptozotocin (STZ) induced diabetic rats. HFD was fed initially to rats which facilitates the development of insulin resistance. Thereafter, an injection of STZ (40 mg/kg, i.p.) was given. This resulted in the development of diabetes with elevated fasting glucose and impaired glucose tolerance. After stabilization of blood glucose values, wounds were created by punch biopsy on the dorsal side of the palm of the rat to mimic the diabetic wounds frequently seen in the case of humans. Later, the application of Botroclot on these wounds was carried out for 15 days. Topical application of hemocoagulase improved the wound closure and there was a gradual decrease in inflammatory markers and a substantial increase in collagen deposition occurred. Histopathological findings indicated the same, with an increase in granulation tissue suggesting that the topical application moderately improves the wound healing in diabetic rats. We conclude that Botroclot can have a mild to moderate effect in improving collagen deposition and thus wound contraction, improving wound closure in diabetic wounds in rats. This study also establishes the basis for exploration of agents from venom-based sources in diabetic wound healing.

Botroclot 是一种含有血凝酶的市售制剂，血凝酶是一种从蛇Botrops atrox 中分离出来的具有凝血活性的酶。该配方用于牙科手术和其他小型手术伤口。然而，该配方尚未在糖尿病伤口中进行测试。因此，我们提出了一项在高脂饮食（HFD）+链脲佐菌素（STZ）诱导的糖尿病大鼠中局部应用 Botroclot 的研究。HFD 最初被喂给大鼠，这促进了胰岛素抵抗的发展。此后，注射STZ（40mg/kg，腹膜内）。这导致了空腹血糖升高和糖耐量受损的糖尿病的发生。血糖值稳定后，通过穿刺活检在大鼠手掌背侧制造伤口，以模仿人类常见的糖尿病伤口。随后，在这些伤口上应用了 Botroclot，持续了 15 天。局部应用血凝酶改善了伤口闭合，炎症标记物逐渐减少，胶原蛋白沉积显着增加。组织病理学结果表明同样的情况，肉芽组织的增加表明局部应用可适度改善糖尿病大鼠的伤口愈合。我们得出的结论是，Botroclot 对改善胶原蛋白沉积、从而改善伤口收缩、改善大鼠糖尿病伤口的伤口闭合具有轻度至中度的作用。这项研究还为探索基于毒液的药物在糖尿病伤口愈合中的应用奠定了基础。