The Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 7 (CHCHD7) gene was located in a growth-related major QTL that participated in the process of bone cells metabolism in animals by regulating mitochondrial copper homeostasis and cytochrome C oxidase assembly. Therefore, we speculated that CHCHD7 gene might be involved in animal growth and body size. Herein, we discovered a 17-bp insertion/deletion (indel) within the goat CHCHD7 gene. Then, we detected this variation in Shaanbei White Cashmere (SBWC, n = 1055) goats and Inner Mongolia White Cashmere (IMWC, n = 743) goats (Alathai type) using the mathematical expectation (ME) method. We then analyzed the correlation between these genotypes and goat body measurement traits. The results showed that the minor allelic frequency (MAF) was 0.011 in SBWC, and 0.048 in IMWC. In SBWC and IMWC, the reaction time by ME method was reduced by 36.78% and 27.59%, respectively, compared to the traditional method of screening samples one by one. Moreover, in SBWC goats, the 17-bp indel was significantly associated with body measurement traits (e.g. body height, and body length) in adults. In IMWC goats, the 17-bp indel was correlated with body measurement traits (e.g. body height) in weaners. In SBWC and IMWC goat populations, the body measurement traits of the individuals homozygous for 17-bp indel were higher than those in heterozygous individuals, except for the case of cannon circumference in IMWC weaners. These findings showed that the 17-bp insertion mutation within the goat CHCHD7 gene significantly affected body morphometric traits, and could provide a basis for marker-assisted selection (MAS) breeding of cashmere goats.

Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 7 ( CHCHD7 ) 基因位于生长相关的主要 QTL 中，通过调节线粒体铜稳态和细胞色素 C 氧化酶组装参与动物骨细胞代谢过程。因此，我们推测CHCHD7基因可能与动物的生长和体型有关。在此，我们在山羊CHCHD7基因内发现了 17 bp 插入/缺失 (indel)。然后，我们使用数学期望（ME）方法检测了陕北白绒山羊（SBWC， n = 1055）和内蒙古白绒山羊（IMWC， n = 743）山羊（阿拉泰型）的这种变异。然后我们分析了这些基因型与山羊身体测量特征之间的相关性。结果表明，SBWC 中次要等位基因频率（MAF）为 0.011，IMWC 中为 0.048。在SBWC和IMWC中，与传统的逐一筛选样品的方法相比，ME方法的反应时间分别缩短了36.78%和27.59%。此外，在 SBWC 山羊中，17 bp 插入缺失与成年山羊的身体测量特征（例如身高和体长）显着相关。在 IMWC 山羊中，17 bp 插入缺失与断奶仔猪的身体测量特征（例如身高）相关。在SBWC和IMWC山羊群体中，除IMWC断奶仔猪的大炮周长外，17-bp插入缺失纯合个体的体形测量性状均高于杂合个体。研究结果表明，山羊CHCHD7基因内的17 bp插入突变显着影响体形态性状，可为绒山羊标记辅助选择（MAS）育种提供依据。