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Genome-wide microsatellites and species specific markers in genus Phytophthora revealed through whole genome analysis
3 Biotech ( IF 2.6 ) Pub Date : 2020-09-18 , DOI: 10.1007/s13205-020-02430-y
Deepu Mathew 1 , P S Anju 1 , Amala Tom 1 , Neethu Johnson 1 , M Lidia George 1 , Sangeetha P Davis 1 , V Ravisankar 1 , K N Asha 1
Affiliation  

Genome wide microsatellite maps shall support Phytophthora systematics through the development of reliable markers, enabling species discrimination and variability analyses. Whole genome sequences of 17 Phytophthora accessions belonging to 14 species were retrieved from GenBank and the genome-wide microsatellites in each species were mined. A total of 51,200 microsatellites, including dinucleotide to decanucleotide motifs, have been identified across all the species and each one was characterized for uniqueness and repeat number. The P. infestans T30-4 genome had the highest (6873) and P. multivora 3378 had the lowest number of microsatellites (1802). Dinucleotide motifs (63.6%) followed by trinucleotide motifs (30.1%) were most abundant in all the genome. From 14 species, 250 microsatellites which are unique for the respective genomes are detailed along with their primer combinations and product sizes. P. sojae had the highest number of unique microsatellite motifs. Genome wide microsatellite maps for all the 14 Phytophthora species including the chromosome, position, motif, repeat number, forward and reverse primer sequences and expected PCR product size, for every microsatellite are presented. Markers based on the unique microsatellites could be used to identify each species, whereas the ones common to all species could be used to identify the genetic variability. Furthermore, to confirm the results, pure cultures of P. capsici, P. nicotianae and P. palmivora were procured from the Phytophthora Repository, DNA was isolated and the unique markers were screened across the species. The characteristic markers developed have confirmed the genome analysis results.



中文翻译:

通过全基因组分析揭示疫霉属的全基因组微卫星和物种特异性标记

全基因组微卫星图应通过开发可靠的标记来支持疫霉属系统学,从而实现物种区分和变异性分析。从 GenBank 检索了属于 14 个物种的 17 个疫霉属植物的全基因组序列,并挖掘了每个物种的全基因组微卫星。已在所有物种中鉴定出总共 51,200 个微卫星,包括二核苷酸到十核苷酸基序,并且每个都具有独特性和重复数量的特征。将马铃薯晚疫病菌T30-4基因组有最高的(6873)和P. multivora3378 颗微卫星数量最少(1802 颗)。二核苷酸基序 (63.6%) 其次是三核苷酸基序 (30.1%) 在所有基因组中最丰富。详细介绍了来自 14 个物种的 250 颗各自基因组独特的微卫星及其引物组合和产物大小。P. sojae具有最多数量的独特微卫星图案。所有 14 种疫霉属的全基因组微卫星图提供了每个微卫星的物种,包括染色体、位置、基序、重复数、正向和反向引物序列以及预期的 PCR 产物大小。基于独特微卫星的标记可用于识别每个物种,而所有物种共有的标记可用于识别遗传变异性。此外,为了证实结果,纯培养辣椒疫霉P.烟草P. palmivora是从采购库,分离DNA和独特的标记物跨物种筛选。开发的特征标记证实了基因组分析结果。

更新日期:2020-09-20
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