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Immunohistochemical determination of mTOR pathway molecules in ovaries and uterus in rat estrous cycle stages.
Histology and Histopathology ( IF 2.5 ) Pub Date : 2020-09-17 , DOI: 10.14670/hh-18-258
Gulcin Ekizceli 1 , Sevinc Inan 2 , Gulperi Oktem 3 , Ece Onur 4 , Kemal Ozbilgin 5
Affiliation  

mTOR is a member of the PI3K/Akt/mTOR signaling pathway that participates in cell growth, proliferation, protein synthesis, transcription, angiogenesis, apoptosis and autophagy. mTOR and MAPK pahways are two important key signal pathways which are related to each other. We investigated the role of mTOR and other signaling molecules in rat ovaries and uteruses in stages of the estrous cycle. Young adult female rats were divided into four groups as proestrous, estrous, metestrous and diestrous according to vaginal smears. Immunohistochemical staining of mTORC1, IGF1, PI3K, pAKT1/2/3, ERK1 and pERK1/2 was performed and pAKT1/2/3 and ERK1 were also analyzed using western blotting on ovarian and uterine tissue samples. According to our results, PI3K/Akt/mTOR and ERK/pERK showed an increase in the rat ovulation period. When all the groups were evaluated the immunoreactivities for all of the antibodies were detected in the oocytes, granulosa and theca cells, corpus luteum and stroma of ovary and lamina propria, surface and glandular epithelium of uterus with the strongest observed with anti-ERK1 antibody and then with a decreasing trend with anti-mTORC1, anti-pAkt1/2/3, anti-IGF1, anti-PI3K and anti-pERK1/2 antibodies in the proestrus and estrus stages. Differently from other parts of the ovary, highest antibody expression in the corpus luteum was observed in the metestrous stage. Moreover, the existence of pAKT1/2/3 and ERK1 proteins was confirmed with the Western blotting technique. We suggest that mTOR and mTOR-related ERK signaling molecules may participate in the rat ovulation process.

中文翻译:

大鼠发情周期阶段卵巢和子宫中mTOR通路分子的免疫组织化学测定。

mTOR 是 PI3K/Akt/mTOR 信号通路的成员,参与细胞生长、增殖、蛋白质合成、转录、血管生成、凋亡和自噬。mTOR和MAPK通路是两个相互关联的重要关键信号通路。我们研究了 mTOR 和其他信号分子在动情周期阶段的大鼠卵巢和子宫中的作用。根据阴道涂片将年轻成年雌性大鼠分为发情前期、发情期、发情期和发情期四组。进行了 mTORC1、IGF1、PI3K、pAKT1/2/3、ERK1 和 pERK1/2 的免疫组织化学染色,并且还使用蛋白质印迹法对卵巢和子宫组织样本分析了 pAKT1/2/3 和 ERK1。根据我们的结果,PI3K/Akt/mTOR和ERK/pERK在大鼠排卵期显示出增加。当对所有组进行评估时,在卵母细胞、颗粒细胞和膜细胞、黄体和卵巢基质和固有层、子宫表面和腺上皮中检测到所有抗体的免疫反应性,抗 ERK1 抗体和在发情前期和发情期,抗mTORC1、抗pAkt1/2/3、抗IGF1、抗PI3K和抗pERK1/2抗体呈下降趋势。与卵巢的其他部分不同,在子宫肌期观察到黄体中抗体表达最高。此外,pAKT1 / 2/3 和 ERK1 蛋白的存在通过蛋白质印迹技术得到证实。我们认为 mTOR 和 mTOR 相关的 ERK 信号分子可能参与了大鼠的排卵过程。
更新日期:2020-09-21
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