In this study, the whole mitochondrial genomes (mitogenomes) from four species were sequenced. The complete mitochondrial genomes of Sinopodisma pieli, S. houshana, S. qinlingensis, and S. wulingshanensis are 15,857 bp, 15,818 bp, 15,843 bp, and 15,872 bp in size, respectively. The 13 protein-coding genes (PCGs) begin with typical ATN codons, except for COXI in S. qinlingensis, which begins with ACC. The highest A+T content in all the sequenced orthopteran mitogenomes is 76.8% (S. qinlingensis), followed by 76.5% (S. wulingshanensis), 76.4% (S. pieli) and 76.4% (S. houshana) (measured on the major strand). The long polythymine stretches (T-stretch) in the A+T-rich region of the four species are not adjacent to the trnI locus but are inside the stem-loop sequences on the major strand. Moreover, several repeated elements are found in the A+T-rich region of the four species. Phylogenetic analysis based on 53 mitochondrial genomes using Bayesian Inference (BI) and Maximum Likelihood (ML) revealed that Melanoplinae (Podismini) was a monophyletic group; however, the monophyly of Sinopodisma was not supported. These data will provide important information for a better understanding of the phylogenetic relationship of Melanoplinae.

中文翻译：

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中华足目四种物种的线粒体基因组比较及其系统发育意义（直翅目、黑眼亚科）


在这项研究中，对四个物种的整个线粒体基因组（线粒体基因组）进行了测序。 Sinpodismapieri、S.houshanensis、S.qinlingensis 和 S.wulingshanensis 的完整线粒体基因组大小分别为 15,857 bp、15,818 bp、15,843 bp 和 15,872 bp。这 13 个蛋白质编码基因 (PCG) 均以典型的 ATN 密码子开头，但秦岭链鱼中的 COXI 则以 ACC 开头。所有已测序的直翅目有丝分裂基因组中 A+T 含量最高，为 76.8%（秦岭 S. Qinlingensis），其次是 76.5%（S. Wulingshanensis）、76.4%（S. Pieli）和 76.4%（S. Houshanensis）（在主链）。四个物种的 A+T 富含区域中的长多聚胸腺嘧啶延伸（T 延伸）不与 trnI 基因座相邻，而是位于主链上的茎环序列内部。此外，在四个物种的 A+T 丰富区域中发现了几个重复元素。使用贝叶斯推理 (BI) 和最大似然 (ML) 基于 53 个线粒体基因组的系统发育分析表明，Melanoplinae (Podismini) 是一个单系群；然而，Sinopodisma 的单一性并未得到支持。这些数据将为更好地理解Melanoplinae的系统发育关系提供重要信息。