We have previously found there was a small subpopulation of cells with cancer stem cell-like phenotype ALDH-1 in cervical cancer. Radiotherapy has been applied in most of the cervical cancer. However,the mechanisms underlying radioresistance still remained elusive. Our study is to explore whether ALDH+ cell promotes radioresistance by hypoxia. Cells were respectively cultured in hypoxia and normoxia environment and analyzed for marker stability, and cell cycle distribution. Results: Cell growth, apoptosis, cell cycle, sphere formation were affected by hypoxia. ALDH-1 and CHK2 were upregulated after hypoxia. Here we show that ALDH-1 positive cells contribute to cervical carcinoma radioresistance through preferential activation of the DNA damage checkpoint response and an increase in DNA repair capacity. The fraction of these cells is enriched after radiation in cervical carcinoma.

中文翻译：

---

在宫颈癌中，ALDH-1阳性细胞表现出的抗辐射表型随着缺氧而增强。

我们先前发现子宫颈癌中有一小部分具有癌症干细胞样表型ALDH-1的细胞。放射疗法已应用于大多数宫颈癌。然而，抗辐射性的机制仍然难以捉摸。我们的研究是探索ALDH +细胞是否通过缺氧促进放射抵抗。将细胞分别在缺氧和常氧环境中培养，并分析标记物的稳定性和细胞周期分布。结果：缺氧影响细胞生长，凋亡，细胞周期，球形成。缺氧后ALDH-1和CHK2上调。在这里，我们显示ALDH-1阳性细胞通过优先激活DNA损伤检查点反应和增加DNA修复能力而有助于宫颈癌的放射抵抗。