The root of a ginseng-like plant named Codonopsis javanica is a valuable ingredient in folk medicine with diverse biological properties and has been used in treatments for various diseases, including leukemia, inflammation, and hepatitis. This study aimed to optimize various parameters related to the extraction process of C. javanica root (CJR) with respect to total phenolic content (TPC), total flavonoid content, and antioxidant activities of the obtained CJR extract. We first performed a series of single-factor investigations taking drying conditions and different extraction parameters such as material size, extraction solvent, solvent concentration, extraction temperature, material/solvent ratio, extraction cycle, and stirring speed as varying factors. Then, a response surface methodology procedure was adopted with a central composite design to optimize further the extraction process in order to maximize the TPC. We found that the use of convection drying at 70 °C for 8 hours gave the extract with the highest TPC and antioxidant activities. Optimal extraction parameters were found as follows: ethanol with a concentration of 56.0% as the solvent, material-to-solvent ratio of 1/38.0 g/mL, extraction time of 67.2 minutes, material size ≤0.5 mm, temperature 60 °C, through 1 extraction cycle, and with a stirring speed of 300 rpm. Under the optimized conditions, the experimental value for TPC was 2.9 mg gallic acid equivalent (GAE)/g dry weight (DW), which is reasonably close to the value predicted by the model (2.8 mg GAE/g DW). The half-maximal inhibitory concentration (IC₅₀) values determined by the 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid free radical tests of the CJR extract obtained under optimal conditions were 1042.3 and 299.0 µg/mL, respectively.

一种名为人参党参的人参类植物的根，在民间医学中具有多种生物学特性，是一种有价值的成分，已被用于治疗多种疾病，包括白血病，炎症和肝炎。本研究旨在优化与爪哇茶提取过程相关的各种参数根（CJR）相对于所得CJR提取物的总酚含量（TPC），总黄酮含量和抗氧化活性。我们首先进行了一系列的单因素研究，将干燥条件和不同的萃取参数（例如物料大小，萃取溶剂，溶剂浓度，萃取温度，物料/溶剂比，萃取周期和搅拌速度）作为变化因素进行了研究。然后，采用响应面方法程序，并采用中央复合设计，以进一步优化提取过程，从而最大化TPC。我们发现，在70°C下使用对流干燥8小时可使提取物具有最高的TPC和抗氧化活性。最佳提取参数如下：以浓度为56.0％的乙醇为溶剂，物料与溶剂的比例为1 / 38.0 g / mL，萃取时间为67.2分钟，物料尺寸≤0.5mm，温度为60°C，经过1个萃取循环，搅拌速度为300 rpm。在最佳条件下，TPC的实验值为2.9 mg没食子酸当量（GAE）/ g干重（DW），这与模型预测的值（2.8 mg GAE / g DW）相当接近。半最大抑制浓度（IC在最佳条件下获得的CJR提取物的2,2-二苯基-1-吡啶并肼基和2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）自由基测定的₅₀）值为1042.3和299.0 µg / mL，分别。