Citrus hystrix DC (kaffir lime) leaf oil exhibited antifungal activities against Aspergillus niger and Candida albicans. This study aimed to evaluate the antifungal activity of kaffir lime leaf oil and microemulsions containing kaffir lime oil against Trichophyton mentagrophytes var. interdigitale. The chemical components of kaffir lime leaf oil were analyzed by gas chromatography coupled with mass spectrometry. Microemulsions containing kaffir lime oil were formulated using Tween 80, propylene glycol, and water using a phase titration method. The microemulsion of kaffir lime leaf oil was evaluated for droplet size, polydispersity index, and zeta potential using a dynamic light scattering technique. The antifungal activities of kaffir lime oil and its microemulsion were investigated through macrodilution and agar well diffusion methods, respectively. The degradation of citronellal in the microemulsion was analyzed by validated UV-Visible spectrophotometry. The minimum inhibitory concentration value of kaffir lime oil was 1.08 ± 0.00 mg/mL. The microemulsion of kaffir lime leaf oil exhibited potent antifungal activity against T. mentagrophytes var. interdigitale. The size, polydispersity index, and zeta potential of freshly prepared microemulsion were 12.82 ± 0.40 nm, 0.183 ± 0.072, and −7.87 ± 0.06 mV, respectively. The microemulsion of kaffir lime leaf oil also demonstrated good physical and chemical stability at specific temperatures. The kaffir lime oil microemulsion was highly stable when stored at 4 °C and 30 °C for 1 month but was unstable at 45 °C. The microemulsion of kaffir lime leaf oil may be an alternative therapeutic against tinea pedis caused by T. mentagrophytes var. interdigitale.

柑桔DC（非洲青柠）叶油对黑曲霉和白色念珠菌具有抗真菌活性。这项研究旨在评估非洲青柠叶油和含有非洲青柠油的微乳液对Trichophyton mentagrophytes var的抗真菌活性。叉指。青柠叶油的化学成分通过气相色谱-质谱联用分析。使用Tween 80，丙二醇和水通过相滴定法配制含有非洲青柠油的微乳液。使用动态光散射技术评估非洲青柠叶油的微乳液的液滴大小，多分散指数和ζ电位。通过宏观稀释法和琼脂井扩散法分别研究了非洲青柠油及其微乳的抑菌活性。通过验证的紫外可见分光光度法分析了微乳液中香茅醛的降解。柠檬青柠油的最小抑菌浓度值为1.08±0.00 mg / mL。柠檬青柠叶油的微乳液显示出有效的抗真菌活性T.mentagrophytes var。叉指。新鲜制备的微乳液的大小，多分散指数和ζ电势分别为12.82±0.40nm，0.183±0.072和-7.87±0.06mV。柠檬青柠叶油的微乳液在特定温度下也表现出良好的物理和化学稳定性。柠檬青柠油微乳液在4°C和30°C下储存1个月时非常稳定，但在45°C下不稳定。柠檬青柠叶油的微乳剂可能是针对由棉铃虫变种T. mentagrophytes var引起的足癣的替代疗法。叉指。