In-stent restenosis (ISR) is one of the main complications in patients undergoing percutaneous coronary angioplasty, and microRNAs participate in the contractile-to-synthetic phenotypic switch of vascular smooth muscle cells, a hallmark of restenosis development. MicroRNAs (miRNAs) can be released into circulation from injured tissues, enticing a potential role as noninvasive biomarkers. We aimed to evaluate circulating levels of miRNA-23b, miRNA-143, and miRNA-145 as diagnostic markers of ISR. 142 patients with coronary artery disease undergoing successful angioplasty and a follow-up angiography were included. Subjects were classified according to the degree of obstruction at the angioplasty site into cases (≥50%) or controls (<50%). Total RNA was isolated from plasma to quantify circulating miRNAs levels, and the ROC curves were constructed. Among circulating miRNAs assessed, miRNA-23b and miRNA-143 were significantly lower in cases (miRNA-23b: and miRNA-143: ) than controls (miRNA-23b: , ; miRNA-143: , ). Plasma levels of miRNA-145 showed no significant differences. The analysis of the ROC curves showed an area under the curve for miRNA-23b of 0.71 (95% CI: 0.62-0.80, ) and 0.69 for miRNA-143 (95% CI: 0.60-0.78; ). Our data suggest that plasma levels of miRNA-23b and miRNA-143 could be useful as noninvasive biomarkers of ISR.

中文翻译：

---

循环中的miRNA-23b和miRNA-143是支架内再狭窄的潜在生物标志物

支架内再狭窄（ISR）是进行经皮冠状动脉成形术的患者的主要并发症之一，microRNA参与了血管平滑肌细胞从收缩到合成的表型转换，这是再狭窄发展的标志。MicroRNA（miRNA）可以从受伤的组织释放到循环系统中，从而具有潜在的非侵入性生物标记作用。我们旨在评估作为ISR诊断标记的miRNA-23b，miRNA-143和miRNA-145的循环水平。包括142例成功接受了血管成形术和随访血管造影的冠状动脉疾病患者。根据血管成形术部位的阻塞程度将受试者分为病例（≥50％）或对照组（<50％）。从血浆中分离出总RNA以定量循环miRNA的水平，并构建了ROC曲线。和miRNA-143： ）比对照组（的miRNA-23B： ， ; miRNA-143 ：、 ）。miRNA-145的血浆水平无明显差异。ROC曲线的分析显示，miRNA-23b的曲线下面积为0.71（95％CI：0.62-0.80，）和miRNA-143的0.69（95％CI：0.60-0.78;）。我们的数据表明，miRNA-23b和miRNA-143的血浆水平可用作ISR的非侵入性生物标志物。