The aim of this study was to observe the expression of miR-9, miR-21, miR-27b, and miR-34a related with E6/E7 in HPV16-, HPV52-, and HPV58-infected cervical cancer patients and explore their possible role in cervical cancer with HPV infection. The expression levels of 4 miRNAs were detected in cervical exfoliated cells using qRT-PCR. In the current study, miR-34a expression was significantly upregulated in HPV-positive cervical cancer compared with the HPV-negative healthy population and HPV-positive CIN, but just the expression of miR-34a in HPV16 cervical cancer was statistically significant, and the expression of HPV52 and HPV58 was not statistically significant. The expression of miR-21 increased in HPV-positive cervical cancer compared with HPV-positive CIN, but only HPV16-infected cervical cancer had statistical significance compared with HPV16-infected CIN. By observing the change trend of each subtype group, we can show that the expression of miR-9 in HPV16 CIN was opposite to the other subtypes, and it was upregulated, compared with HPV58 CIN, and significantly increased. The level change of miR-27b in HPV58 cervical cancer and HPV58 CIN was opposite to the other subtypes; unlike the expression of miR-27b which was upregulated in HPV16 and HPV52 infected, it was downregulated compared with Normal. We also found that the expression of miR-34a and miR-9 was contrary to other studies. These findings indicate that the upregulated miR-21 expression may be a biomarker to distinguish between CC and CIN. miR-34a in HPV infection, especially in HPV16 infection, might be related to the occurrence and development of cervical cancer. The infection of different subtypes may play different roles in disease by activating different mechanisms; miRNAs play a very complex role in tumorigenesis and development, and there may be multiple targets in which multiple mechanisms play a role.

中文翻译：

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HPV16 / 58 / 52-感染的宫颈癌中miR-9，miR-21，miR-27b和miR-34a的表达

这项研究的目的是观察感染HPV16，HPV52和HPV58的宫颈癌患者中与E6 / E7相关的miR-9，miR-21，miR-27b和miR-34a的表达，并探讨其可能在HPV感染子宫颈癌中的作用。使用qRT-PCR检测宫颈脱落细胞中4种miRNA的表达水平。在当前研究中，与HPV阴性健康人群和HPV阳性CIN相比，HPV阳性宫颈癌中miR-34a的表达明显上调，但在HPV16宫颈癌中miR-34a的表达具有统计学意义，并且HPV52和HPV58的表达无统计学意义。与HPV阳性CIN相比，HPV阳性宫颈癌中miR-21的表达增加，但只有HPV16感染的子宫颈癌与HPV16感染的CIN相比有统计学意义。通过观察每个亚型组的变化趋势，我们可以发现miR-9在HPV16 CIN中的表达与其他亚型相反，并且与HPV58 CIN相比表达上调，并且显着增加。HPV58宫颈癌和HPV58 CIN中miR-27b的水平变化与其他亚型相反。与在HPV16和HPV52感染后miR-27b的表达上调不同，与正常相比，miR-27b的表达下调。我们还发现miR-34a和miR-9的表达与其他研究相反。这些发现表明，miR-21表达上调可能是区分CC和CIN的生物标记。miR-34a在HPV感染中，尤其是在HPV16感染中，可能与子宫颈癌的发生和发展有关。通过激活不同的机制，不同亚型的感染可能在疾病中扮演不同的角色。miRNA在肿瘤的发生和发展中起着非常复杂的作用，并且可能在多个靶标中多种机制起作用。