Elucidating the Membrane Dynamics and Encapsulation Mechanism of Large DNA Molecules Under Molecular Crowding Condition Using Giant Unilamellar Vesicles.,ACS Synthetic Biology

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Elucidating the Membrane Dynamics and Encapsulation Mechanism of Large DNA Molecules Under Molecular Crowding Condition Using Giant Unilamellar Vesicles.
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2020-09-16 , DOI: 10.1021/acssynbio.0c00360
Mamiko Tsugane ₁ , Hiroaki Suzuki ₁

Affiliation

The conservation throughout evolution of membrane-bound structures that encapsulate genomic material indicates the existence of a simple, physical mechanism that facilitates the enclosing of long-stranded DNA by lipid bilayers. This study aimed to elucidate such a mechanism by investigating how molecular crowding promotes the spontaneous enveloping of model DNA into lipid bilayer membranes. Using fluorescence microscopy and giant unilamellar vesicles (GUVs) we showed that a 166 kb DNA molecule coencapsulated with a model crowder attaches to the inner membrane of the GUVs as they osmotically deflate and after the DNA–membrane complex buds out. The set of results is consistent with the hypothesis that the depletion volume effect is responsible for the spontaneous encapsulation of DNA in the GUVs. This phenomenon may offer novel insights into the basic mechanisms governing membrane encapsulation of long-stranded nucleic acids found in celluar sytems that are independent of genetic control.

中文翻译：

使用巨型单层囊泡阐明分子拥挤条件下大 DNA 分子的膜动力学和封装机制。

封装基因组材料的膜结合结构在整个进化过程中的保守性表明存在一种简单的物理机制，该机制有助于脂质双层包裹长链 DNA。本研究旨在通过研究分子拥挤如何促进模型 DNA 自发包裹到脂质双层膜中来阐明这种机制。使用荧光显微镜和巨大的单层囊泡 (GUV)，我们发现一个 166 kb 的 DNA 分子与模型拥挤的共包囊物附着在 GUV 的内膜上，因为它们渗透性放气，并且在 DNA-膜复合物出芽后。这组结果与消耗体积效应是导致 GUV 中 DNA 自发封装的假设一致。

更新日期：2020-10-17

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