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Dopamine/Polyethylenimine-Modified Silica for Enzyme Immobilization and Strengthening of Enzymatic CO2 Conversion
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2020-09-17 , DOI: 10.1021/acssuschemeng.0c04975 Tingting Zhai 1 , Caihong Wang 1 , Fengjuan Gu 1 , Zi-hui Meng 1 , Wenfang Liu 1 , Yanzi Wang 2
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2020-09-17 , DOI: 10.1021/acssuschemeng.0c04975 Tingting Zhai 1 , Caihong Wang 1 , Fengjuan Gu 1 , Zi-hui Meng 1 , Wenfang Liu 1 , Yanzi Wang 2
Affiliation
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Enzymatic conversion of CO2 to high-value chemicals is a green route of resource reutilization, which is limited by low solubility of CO2 in mild conditions. Here, silica microspheres were first modified by the co-deposition of polyethyleneimine (PEI) and dopamine and the resultant polydopamine (PDA)/PEI-SiO2 was then used as the strengthening material of the enzymatic conversion of CO2 to formate. The initial reaction rate increased from 13.4 to 27.2 times with the loading amount of PDA/PEI-SiO2 increasing from 0.1 to 0.4 g. Following that, PDA/PEI-SiO2 was used as the carrier for the immobilization of carbonic anhydrase (CA). As a result of synergism, the formate synthesis was accelerated up to 48.6 times with a low loading amount of 0.2 g of PDA/PEI-SiO2-CA. Although the promoting effect of PDA/PEI-SiO2 retained only 56.1% after being used once, PDA/PEI-SiO2-CA retained 95.1% activity after 10 cycles of use. It was presumed that CA boosted the impact and reusability of PDA/PEI-SiO2 by improving CO2 desorption. This work is much significant in providing a simple and high-efficiency strategy for process intensification of enzymatic CO2 conversion.
中文翻译：
多巴胺/聚乙烯亚胺改性的二氧化硅用于固定化酶和增强酶促CO 2转化
将CO 2酶转化为高价值化学品是资源再利用的绿色途径，这受限于温和条件下CO 2的低溶解度。在此，首先通过聚乙烯亚胺（PEI）和多巴胺的共沉积来改性二氧化硅微球，然后将所得的聚多巴胺（PDA）/ PEI-SiO 2用作CO 2酶促转化为甲酸酯的强化材料。初始反应速率从13.4倍增加到27.2倍，而PDA / PEI-SiO 2的负载量从0.1克增加到0.4克。之后，PDA / PEI-SiO 2将其用作固定碳酸酐酶（CA）的载体。协同作用的结果是，在0.2g PDA / PEI-SiO 2 -CA的低负载量下，甲酸的合成被加速至48.6倍。一次使用后，虽然PDA / PEI-SiO 2的促进效果仅保持56.1％，但使用10次循环后，PDA / PEI-SiO 2 -CA的活性保持95.1％。据推测，CA通过改善CO 2的解吸作用而增强了PDA / PEI-SiO 2的影响和可重复使用性。这项工作对于为酶促CO 2转化过程的强化提供一个简单而高效的策略非常重要。
更新日期：2020-10-12
中文翻译：
多巴胺/聚乙烯亚胺改性的二氧化硅用于固定化酶和增强酶促CO 2转化
将CO 2酶转化为高价值化学品是资源再利用的绿色途径，这受限于温和条件下CO 2的低溶解度。在此，首先通过聚乙烯亚胺（PEI）和多巴胺的共沉积来改性二氧化硅微球，然后将所得的聚多巴胺（PDA）/ PEI-SiO 2用作CO 2酶促转化为甲酸酯的强化材料。初始反应速率从13.4倍增加到27.2倍，而PDA / PEI-SiO 2的负载量从0.1克增加到0.4克。之后，PDA / PEI-SiO 2将其用作固定碳酸酐酶（CA）的载体。协同作用的结果是，在0.2g PDA / PEI-SiO 2 -CA的低负载量下，甲酸的合成被加速至48.6倍。一次使用后，虽然PDA / PEI-SiO 2的促进效果仅保持56.1％，但使用10次循环后，PDA / PEI-SiO 2 -CA的活性保持95.1％。据推测，CA通过改善CO 2的解吸作用而增强了PDA / PEI-SiO 2的影响和可重复使用性。这项工作对于为酶促CO 2转化过程的强化提供一个简单而高效的策略非常重要。
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