Plasmodium vivax has two invasion ligand/host receptor pathways (PvDBP/DARC and PvRBP2b/TfR1) that are promising targets for therapeutic intervention. We optimized invasion assays with isogenic cultured reticulocytes. Using a receptor blockade approach with multiple P. vivax isolates, we found that all strains utilized both DARC and TfR1, however with significant variation in receptor usage. This suggests that P. vivax, like P. falciparum, uses alternative invasion pathways with implications for pathogenesis and vaccine development.

中文翻译：

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间日疟原虫菌株使用替代途径进行入侵。

间日疟原虫有两种入侵配体/宿主受体途径（PvDBP/DARC 和 PvRBP2b/TfR1），它们是治疗干预的有希望的靶点。我们使用等基因培养的网织红细胞优化了入侵检测。使用多个间日疟原虫分离株的受体阻断方法，我们发现所有菌株都利用了 DARC 和 TfR1，但受体的使用存在显着差异。这表明间日疟原虫与恶性疟原虫一样，使用对发病机制和疫苗开发有影响的替代入侵途径。