A meridic diet overlay bioassay using empty, positively charged zein nanoparticles ((+)ZNP) was performed on soybean looper (Chrysodeixis includens (Walker)), tobacco budworm (Heliothis virescens (F.)), and velvetbean caterpillar (Anticarsia gemmatalis Hübner) (Lepidoptera: Noctuidae). Assessment of effects on mortality and development weights 7 d after ingestion of (+)ZNP were evaluated on larvae of each species. Treatments involved different concentrations, with H. virescens and A. gemmatalis offered 0 and 3,800 ppm (+)ZNP, whereas C. includens colonies were offered 0, 630, 1,260, and 2,520 ppm (+)ZNP. Mortality of A. gemmatalis and C. includens increased after ingestion of the highest (+)ZNP concentrations, while H. virescens neonate mortality was unaffected. Neonate and third-instar weights of A. gemmatalis and C. includens, and neonate H. virescens, decreased with high (+)ZNP concentrations. Following mortality results from A. gemmatalis neonates, a concentration response test was performed using a range of (+)ZNP concentrations. The LC50 for A. gemmatalis was 1,478 ppm. The potential of (+)ZNP as a pest management tactic is discussed.

中文翻译：

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阐明摄入的带正电荷的玉米醇溶蛋白纳米颗粒对抗夜蛾的功效


使用空的、带正电荷的玉米醇溶蛋白纳米颗粒 ((+)ZNP) 对大豆尺蠖 (Chrysodeixis includens (Walker))、烟草芽虫 (Heliothis virescens (F.)) 和豆毛毛虫 (Anticarsia gemmatalis Hübner) 进行了夏季饮食覆盖生物测定。 （鳞翅目：夜蛾科）。摄入(+)ZNP 7天后对每个物种的幼虫的死亡率和发育重量的影响进行评估。处理涉及不同的浓度，H. virescens 和 A. gemmatalis 提供了 0 和 3,800 ppm (+)ZNP，而 C. includens 菌落提供了 0、630、1,260 和 2,520 ppm (+)ZNP。摄入最高浓度的 (+)ZNP 后，A. gemmatalis 和 C. includens 的死亡率增加，而 H. virescens 新生儿死亡率不受影响。 A. gemmatalis 和 C. includens 以及新生儿 H. virescens 的新生儿和三龄体重随着 (+)ZNP 浓度的升高而降低。根据 A. gemmatalis 新生儿的死亡率结果，使用一系列 (+)ZNP 浓度进行浓度响应测试。 A. gemmatalis 的 LC50 为 1,478 ppm。讨论了 (+)ZNP 作为害虫管理策略的潜力。