Cystic echinococcosis (CE) is one of the most widespread zoonotic diseases, with considerable public health and economic importance. Camels play a significant role in transmission cycle of Echinococcus granulosus especially, in the Middle East and North Africa (MENA). The present study aimed to identify the genetic variation and haplotype distribution of camel isolates of E. granulosus sensu lato using all existing E. granulosus mitochondrial DNA data from camels in different parts of the world. Sequence data from 1,144 camel isolates of E. granulosus s.l. available in the NCBI GenBank including 57 camel hydatid cysts collected in central Iran were used to analyze the nature of genetic variation within the camel isolates of E. granulosus s.l. in MENA region. Fifty-seven camel isolates were also PCR-sequenced on mitochondrial 12S rRNA gene. Haplotype network analysis revealed seven different haplotypes clustered into four major groups. E. intermedius G6 was identified as the most commonly represented genotype in camels followed by G1. Mitochondrial 12S rRNA gene sequence analysis on 57 camel isolates identified three different genotypes, including E. intermedius/G6 (35/57, 61.4%), E. granulosus sensu stricto/G1-G3 (21/57, 36.8%) as well as one isolate identified as E. ortleppi/G5 (1/57, 1.8%). The number of base substitutions per site over 420 positions of partial 12S rRNA gene sequences were shown as 0.000 and 0.004 for E. intermedius (G6) corresponding to the Middle East and sub-Saharan isolates, respectively. Camel isolates of E. granulosus in the MENA region present moderate genetic diversity (Hd = 0.5540–0.6050). The Middle East isolates demonstrated a more diverse population than the North/sub-Saharan isolates, where six out of seven 12S rRNA haplotypes were identified in the former region. E. intermedius (G6 genotype) was shown to be the most common species in the world camel population. In conclusion, camels showed to be an important intermediate host species in the MENA region with different patterns of genetic variation between the Middle East and Africa.

囊性棘球co病（CE）是最广泛的人畜共患病之一，具有相当大的公共卫生和经济意义。骆驼在细粒棘球oc特别是在中东和北非（MENA）。本研究旨在确定骆驼分离物的遗传变异和单倍型分布。颗粒大肠杆菌 sensu lato使用所有现有的 颗粒大肠杆菌来自世界各地的骆驼的线粒体DNA数据。来自1,144株骆驼科分离物的序列数据颗粒大肠杆菌 NCBI GenBank中提供的sl包括在伊朗中部收集的57个骆驼包虫囊肿用于分析美洲驼骆驼分离物中遗传变异的性质。 颗粒大肠杆菌中东和北非地区的sl。还对线粒体12S rRNA基因进行了PCR测序，对57个骆驼分离物进行了测序。单倍型网络分析揭示了七个不同的单倍型，分为四个主要组。中间EG6被确定为骆驼中最常见的基因型，其次是G1。对57个骆驼分离物的线粒体12S rRNA基因序列分析确定了三种不同的基因型，包括中间E/ G6（35/57，61.4％）， 颗粒大肠杆菌 sensu stricto / G1-G3（21/57，36.8％）以及一种鉴定为 大肠杆菌/ G5（1/57，1.8％）。对于12S rRNA部分基因序列的420个位置，每个位点的碱基取代数分别为0.000和0.004中间E（G6）分别对应于中东和撒哈拉以南的分离株。骆驼的分离物颗粒大肠杆菌在中东和北非地区目前存在中等遗传多样性（Hd = 0.5540–0.6050）。中东分离株显示出比北部/撒哈拉以南分离株更多样化的种群，后者在前一个地区鉴定出7种12S rRNA单倍型。中间E（G6基因型）被证明是世界骆驼种群中最常见的物种。总之，骆驼是中东和非洲地区中东和非洲地区重要的中间寄主物种，具有不同的遗传变异模式。