Antimicrobial peptides (AMPs) play an important role in innate immunity in human skin. It is known that AMPs mainly function in the stratum corneum. Therefore, AMP concentrations in the stratum corneum need to be precisely measured to clarify functional and physiological importance of AMPs in cutaneous defence. Tape stripping (TS) is a well-established method by which components in the stratum corneum can be collected. However, the usefulness of the TS method for measuring AMP concentration in human skin remains unclear. Therefore, we compared it with another popular method, skin rinsing, which had been established as a method for measuring AMP concentration in human skin. When investigated on healthy medial forearm using RNase 7, which is one of the typical AMPs, as an index, there was a significant positive correlation between RNase 7 concentrations measured by the TS method at adjacent forearm sites, demonstrating the reproducibility of the TS method. Next, a significant positive correlation was detected in RNase 7 concentrations measured using the TS and the skin rinsing method, indicating that the TS method is comparable to the skin rinsing method. Thus, we speculate that the TS method is useful for measuring AMP concentration in human skin.

抗菌肽 (AMP) 在人类皮肤的先天免疫中发挥重要作用。已知AMPs主要在角质层中起作用。因此，需要精确测量角质层中的 AMP 浓度，以阐明 AMP 在皮肤防御中的功能和生理重要性。胶带剥离 (TS) 是一种成熟的方法，通过该方法可以收集角质层中的成分。然而，TS 方法用于测量人体皮肤中 AMP 浓度的有用性仍不清楚。因此，我们将其与另一种流行的方法——皮肤冲洗——进行了比较，后者已被确立为一种测量人体皮肤中 AMP 浓度的方法。当使用 RNase 7（典型的 AMP 之一）作为指标对健康的前臂内侧进行调查时，在相邻的前臂部位通过 TS 方法测量的 RNase 7 浓度之间存在显着的正相关，证明了 TS 方法的重现性。接下来，在使用 TS 和皮肤冲洗方法测量的 RNase 7 浓度中检测到显着的正相关，表明 TS 方法与皮肤冲洗方法相当。因此，我们推测 TS 方法可用于测量人体皮肤中的 AMP 浓度。