Early postnatal life is characterized by a critical time period in which the developing neonatal immune system transitions from passive immunity, induced by protective maternal antibodies, to the competence of a fully functioning immune system. The inflammatory capability of both maternal and neonatal antibodies is governed by N-linked glycosylation of the Fc region, and though this has been examined extensively in adults, there is currently little information regarding antibody glycosylation patterns during early postnatal life. To characterize the murine IgG Fc glycosylation profile during early life, we used nano-LC-ESI-Qq-TOF mass spectrometry analysis to assess subclass specific Asn-297 glycosylation patterns in the serum of BALB/c mice from 5–60 days of age. From birth to adulthood, we observed a decline in proinflammatory Fc glycosylation in all IgG subclasses. This was shown by significantly reduced agalactosylated and monogalactosylated structures combined with increased sialylation after weaning at 45 and 60 days of age. This information indicates that the transition between neonatal life and adulthood in mice is accompanied by reduction of inflammatory IgG antibodies. Our study contributes to a growing body of literature indicating the importance of IgG Fc glycosylation and its association with inflammation during different life stages.

出生后早期的特点是一个关键时期，在此期间，发育中的新生儿免疫系统从由保护性母体抗体诱导的被动免疫过渡到功能齐全的免疫系统的能力。母体和新生儿抗体的炎症能力都受 Fc 区 N 联糖基化的控制，虽然这已在成人中进行了广泛检查，但目前关于出生后早期抗体糖基化模式的信息很少。为了表征早期小鼠 IgG Fc 糖基化特征，我们使用 nano-LC-ESI-Qq-TOF 质谱分析来评估 5-60 天龄 BALB/c 小鼠血清中亚类特异性 Asn-297 糖基化模式. 从出生到成年，我们观察到所有 IgG 亚类中促炎 Fc 糖基化的下降。这可以通过在 45 和 60 日龄断奶后显着减少的无半乳糖基化和单半乳糖基化结构以及增加的唾液酸化来证明。该信息表明，小鼠从新生儿到成年的过渡伴随着炎症 IgG 抗体的减少。我们的研究有助于越来越多的文献表明 IgG Fc 糖基化的重要性及其在不同生命阶段与炎症的关联。该信息表明，小鼠从新生儿到成年的过渡伴随着炎症 IgG 抗体的减少。我们的研究有助于越来越多的文献表明 IgG Fc 糖基化的重要性及其在不同生命阶段与炎症的关联。该信息表明，小鼠从新生儿到成年的过渡伴随着炎症 IgG 抗体的减少。我们的研究有助于越来越多的文献表明 IgG Fc 糖基化的重要性及其在不同生命阶段与炎症的关联。