Pomegranate seed oil (PSO) is mainly composed of punicic acid (PA), a polyunsaturated fatty acid also known as omega-5 (ω-5), a potent antioxidant associated with a variety of metabolic and cellular beneficial effects. However, the potential benefits of a nanoemulsified version of ω-5 (PSOn) have not been evaluated in a pathological liver condition. Here, we examined whether PSOn had beneficial effects on C57BL/6N mice fed a high-fat diet (HFD), specifically on hepatic steatosis. We observed that PSOn supplementation decreased body weight and body fat mass in control mice, whereas glucose intolerance, insulin resistance, energy expenditure, and hepatic steatosis were improved in both control mice and in mice fed a HFD. Interestingly, PSOn increased fatty acid oxidation in primary hepatocytes and antioxidant gene expression. Altogether, our data indicate that PSOn effectively reduces some of the HFD-derived metabolic syndrome indicators by means of an increase in fatty acid oxidation within hepatocytes.

石榴籽油 (PSO) 主要由石榴酸 (PA) 组成，石榴酸是一种多不饱和脂肪酸，也称为 omega-5 (ω-5)，是一种有效的抗氧化剂，与多种代谢和细胞有益作用相关。然而，纳米乳化形式的 ω-5 (PSOn) 的潜在益处尚未在病理性肝脏疾病中得到评估。在这里，我们检查了 PSOn 是否对高脂饮食 (HFD) 喂养的 C57BL/6N 小鼠有有益作用，特别是对肝脂肪变性。我们观察到补充 PSOn 降低了对照小鼠的体重和体脂肪量，而对照小鼠和喂食 HFD 的小鼠的葡萄糖耐受不良、胰岛素抵抗、能量消耗和肝脂肪变性均得到改善。有趣的是，PSOn 增加了原代肝细胞中的脂肪酸氧化和抗氧化基因表达。总而言之，我们的数据表明，PSOn 通过增加肝细胞内脂肪酸氧化，有效减少一些 HFD 衍生的代谢综合征指标。