Microbicides play critical roles in infection prevention and control of Ebola virus by decontaminating high-touch environmental surfaces (HITES), interrupting the virus-HITES-hands nexus. We evaluated the efficacy of formulations containing different microbicidal actives for inactivating Ebola virus–Makona strain (EBOV/Mak) on stainless-steel carriers per ASTM E2197-11. Formulations of sodium hypochlorite (NaOCl) (0.05–1%), ethanol (70%), chloroxylenol (PCMX) (0.12–0.48% by weight) in hard water, and a ready-to-use disinfectant spray with 58% ethanol (EDS), were tested at contact times of 0, or 0.5 to 10 min at ambient temperature. EBOV/Mak was inactivated (> 6 log₁₀) by 70% ethanol after contact times ≥ 2.5 min, by 0.5% and 1% NaOCl or EDS (> 4 log₁₀) at contact times ≥ 5 min, and by 0.12–0.48% PCMX (> 4.2 log₁₀) at contact times ≥ 5 min. Residual infectious virus in neutralized samples was assessed by passage on cells and evaluation for viral cytopathic effect. No infectious virus was detected in cells inoculated with EBOV/Mak exposed to NaOCl (0.5% or 1%), PCMX (0.12% to 0.48%), or EDS for ≥ 5 min. These results demonstrate ≥ 6 log₁₀ inactivation of EBOV/Mak dried on prototypic surfaces by EDS or formulations of NaOCl (≥ 0.5%), PCMX (≥ 0.12%), or 70% ethanol at contact times ≥ 5 min.

杀菌剂通过净化高接触环境表面 (HITES)、中断病毒-HITES-手的关系，在埃博拉病毒感染预防和控制中发挥着关键作用。我们根据 ASTM E2197-11 评估了含有不同杀菌活性物质的制剂对不锈钢载体上埃博拉病毒 Makona 株 (EBOV/Mak) 的灭活效果。次氯酸钠 (NaOCl) (0.05–1%)、乙醇 (70%)、氯二甲酚 (PCMX)（0.12–0.48%，按重量计）的硬水配方，以及含 58% 乙醇的即用型消毒喷雾剂（ EDS），在环境温度下以 0 或 0.5 至 10 分钟的接触时间进行测试。接触时间 ≥ 2.5 分钟后，EBOV/Mak 被 70% 乙醇灭活 (> 6 log ₁₀ )，接触时间 ≥ 5 分钟时，0.5% 和 1% NaOCl 或 EDS (> 4 log ₁₀ ) 灭活，0.12– 0.48% PCMX (> 4.2 log ₁₀ )，接触时间 ≥ 5 分钟。通过细胞传代和评估病毒细胞病变效应来评估中和样品中残留的感染性病毒。在接种 EBOV/Mak 并暴露于 NaOCl（0.5% 或 1%）、PCMX（0.12% 至 0.48%）或 EDS ≥ 5 分钟的细胞中，未检测到感染性病毒。这些结果表明，在接触时间≥ 5 分钟时，通过 EDS 或 NaOCl (≥ 0.5%)、PCMX (≥ 0.12%) 或 70% 乙醇制剂在原型表面上干燥的 EBOV/Mak 失活≥ 6 log ₁₀ 。