Raman/fluorescence microspectroscopic analysis of individual polymer chains and nanobeads dissolved in solution will become a powerful analytical method to study their molecular structure and characteristics. With this motivation, we focused on the use of Raman microspectroscopy for optically trapped soft matter. A tightly focused near-infrared laser beam formed a microassembly of thermoresponsive polymer chains such as poly(N-isopropylacrylamide) due to a local photothermal effect and optical force. By using this method, we developed a technique for determining the polymer concentration in a polymer microassembly. Furthermore, we demonstrated a molecular condensation and detection technique based on microassembly on plasmonic nanostructures. For this molecular condensation and detection process, localized surface plasmons play an essential role in the optical force enhancement and local temperature increase around the plasmonic nanostructures. Finally, aiming toward novel manipulation methods of smaller soft nanomaterials, nanostructured semiconductor-assisted (NASSCA) optical tweezers are introduced. In this paper, we reviewed the optical manipulation methods of polymer chains and nanobeads and their applications in analytical chemistry. We reviewed our recent studies on optical manipulation techniques for microspectroscopic analysis of optically trapped polymers. A focused laser beam exerts an optical force on polymer chains, leading to the microassembly formation of them. We applied the conventional optical tweezers for the concentration determination techniques of phase-separated thermoresponsive polymer chains by combining with microspectroscopies. To overcome the limitations of the conventional optical tweezers, localized surface plasmon has attracted much attention. Finally, we introduced our original manipulation technique based on nanostructured semiconductor-assisted optical tweezers; NASSCA optical tweezers.

中文翻译：

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用于显微聚合物分析的纳米结构辅助光镊

溶解在溶液中的单个聚合物链和纳米珠的拉曼/荧光显微光谱分析将成为研究其分子结构和特性的有力分析方法。出于这个动机，我们专注于拉曼显微光谱用于光学捕获的软物质。由于局部光热效应和光学力，紧密聚焦的近红外激光束形成了热响应聚合物链的微组装，例如聚（N-异丙基丙烯酰胺）。通过使用这种方法，我们开发了一种用于确定聚合物微组件中聚合物浓度的技术。此外，我们展示了一种基于等离子体纳米结构微组装的分子凝聚和检测技术。对于这个分子凝聚和检测过程，局部表面等离子体在等离子体纳米结构周围的光学力增强和局部温度升高中起着至关重要的作用。最后，针对较小的软纳米材料的新型操作方法，介绍了纳米结构半导体辅助 (NASSCA) 光镊。在本文中，我们回顾了聚合物链和纳米珠的光学操纵方法及其在分析化学中的应用。我们回顾了我们最近对用于光学捕获聚合物的显微光谱分析的光学操作技术的研究。聚焦的激光束对聚合物链施加光学力，导致它们的微组装形成。我们将传统的光镊与显微光谱相结合，用于相分离的热敏聚合物链的浓度测定技术。为了克服传统光镊的局限性，局部表面等离子体引起了广泛关注。最后，我们介绍了基于纳米结构半导体辅助光镊的原始操作技术；NASSCA 光镊。