PEGDMA Hydrogels for Cell Adhesion and Optical Waveguiding,ACS Applied Bio Materials

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PEGDMA Hydrogels for Cell Adhesion and Optical Waveguiding
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-09-17 , DOI: 10.1021/acsabm.0c00885
Sonja Johannsmeier _{1,

2} , Minh Thanh Truc Nguyen ₁ , Ruben Hohndorf ₁ , Gerald Dräger ₃ , Dag Heinemann _{1,

4,

5} , Tammo Ripken _{1,

2} , Alexander Heisterkamp _{1,

2,

6}

Affiliation

Hydrogels are favored materials in tissue engineering as they can be used to imitate tissues, provide scaffolds, and guide cell behavior. Recent advances in the field of optogenetics have created a need for biocompatible optical waveguides, and hydrogels have been investigated to meet these requirements. However, combining favorable waveguiding characteristics, high biocompatibility, and controllable bioactivity in a single device remains challenging. Here, we investigate the use of poly(ethylene glycol) hydrogels as carriers and illumination systems for in vitro cell culture. We present a comprehensive and reproducible protocol for selective bioactivation of the hydrogels, achieving high proliferation rates and strong cell adhesion on the treated surface. A cell model expressing the photoconvertible fluorescent protein Dendra2 confirmed that light-cell interactions occur at the hydrogel surface. Monte Carlo simulations were performed as a tool to predict the extent of these interactions. This study demonstrates a hydrogel-based waveguiding system for targeted cell stimulation in vitro and potentially in vivo environments.

中文翻译：

用于细胞粘附和光波导的 PEGDMA 水凝胶

水凝胶是组织工程中的首选材料，因为它们可用于模拟组织、提供支架和指导细胞行为。光遗传学领域的最新进展已经产生了对生物相容性光波导的需求，并且已经研究了水凝胶以满足这些要求。然而，在单个设备中结合有利的波导特性、高生物相容性和可控的生物活性仍然具有挑战性。在这里，我们研究了聚（乙二醇）水凝胶作为体外载体和照明系统的用途细胞培养。我们提出了一种全面且可重复的方案，用于水凝胶的选择性生物活化，在处理过的表面上实现高增殖率和强细胞粘附。表达光可转换荧光蛋白 Dendra2 的细胞模型证实光细胞相互作用发生在水凝胶表面。蒙特卡罗模拟被用作预测这些相互作用程度的工具。这项研究展示了一种基于水凝胶的波导系统，用于在体外和潜在的体内环境中进行靶向细胞刺激。

更新日期：2020-10-21

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