Abstract

Stachys sieboldii MiQ (SSM) is an important food and medicinal herb in Korea, used to improve memory of patients with senile dementia and cardiovascular diseases. However, little information on bioactive components from SSM or standardized extraction methods for these components is available. This study isolated and purified major components from SSM for the first time, and assessed their ability to inhibit soluble epoxide hydrolase (sEH). The results showed that acteoside is the most potent inhibitor of sEH, with an IC₅₀ of 33.5 ± 0.5 μM. Additional active components, including harpagide, tryptophan, and 8-acetate-harpagide, along with acteoside, were tentatively identified using high‐performance liquid chromatography photodiode array tandem mass spectrometry (HPLC–PDA–MS/MS) and quantified using an ultraviolet detector at 210 nm. Further, an ultrasonic-assisted extraction technique for extraction of four bioactive compounds in SSM was developed and optimized using response surface methodology (RSM). The optimal extraction conditions were: extraction time, 30.46 minutes; extraction temperature, 67.95 °C, and methanol concentration 53.85%. The prediction model of RSM was validated with laboratory experiments. The similarity between predicted and actual values was 97.84%. The extraction method is thus a rapid, environment-friendly, energy-saving method can be applied to extract bioactive components from SSM in large quantities.

摘要

Stachys sieboldii MiQ（SSM）是韩国重要的食品和草药，用于改善老年痴呆症和心血管疾病患者的记忆力。但是，关于来自SSM的生物活性成分或这些成分的标准化提取方法的信息很少。这项研究首次从SSM中分离和纯化了主要成分，并评估了它们抑制可溶性环氧化物水解酶（sEH）的能力。结果表明，Acteoside是最有效的sEH抑制剂，IC ₅₀为33.5±0.5μM。使用高效液相色谱光电二极管阵列串联质谱法（HPLC-PDA-MS / MS）初步鉴定了其他活性成分，包括杀虫肽，色氨酸和8-乙酸酯-杀虫剂，以及杀藻甙，并使用紫外检测器进行定量210纳米 此外，开发了一种超声辅助提取技术，用于提取SSM中的四种生物活性化合物，并使用响应表面方法（RSM）对其进行了优化。最佳提取条件为：提取时间30.46分钟；提取时间30.46分钟。萃取温度67.95°C，甲醇浓度53.85％。通过实验室实验验证了RSM的预测模型。预测值与实际值之间的相似度为97.84％。因此，提取方法是一种快速，环境友好，