Abstract

Aim

Recently, higher contamination by aflatoxin M1 (AFM1) has been detected in many countries. Unfortunately, many tons of contaminated milk and milk byproducts are removed from the food chain to avoid human contamination; as a consequence of higher economic losses. Fewest number of studies are interested to AFM1 detoxification using lactic acid bacteria.

Materials and methods

In this study, AFM1-degradation using Lactobacillus paracasei BEJ01 (LPBEJ01) was tested in vitro. The preventive effect of LPBEJ01 against AFM1 immunobiological effects in mice are treated orally during 3 weeks with 100 µg AFM1, LPBEJ01 (2 × 10⁹ CFU/ml∼2 mg/kg p.c.) and a mixture of AFM1 and LPBEJ01.

Results

In vitro LPBEJ01 was found able to absorb 98% of AFM1 (100 µg/ml) in liquid medium after 24 h and more than 95% of AFM1 could be eliminated after 24 h in a solid-state fermentation. Animals treated with AFM1 obtained lower body weight than the control ones. The mitogenic response of spleen mononuclear cells (SMCs) in vivo was higher in mice treated with AFM1. The SMC of mice treated with AFM1 produced lower levels of IL-2, higher levels IL-4 and no effect on IL-10 production. The peritoneal macrophages of mice that treated with AFM1 released less H₂O₂, while mice exposed orally with the mixture of AFM1 and LPBEJ01 produced higher levels.

Conclusion

LPBEJ01 was safe and it did not have any sign of toxicity. It can be used as an additive for AFM1-detoxification contamination in the food chain in countries suffering from this problem.

中文翻译：

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AFM1在小鼠乳酸菌预防的亚慢性给药实验中的免疫学作用。

摘要

目标

最近，在许多国家中发现了黄曲霉毒素M1（AFM1）造成的更高污染。不幸的是，从食物链中清除了许多吨受污染的牛奶和牛奶副产品，以避免人为污染；由于较高的经济损失。很少有研究对使用乳酸菌进行AFM1解毒感兴趣。

材料和方法

在这项研究中，在体外测试了使用副干酪乳杆菌BEJ01（LPBEJ01）进行AFM1降解的方法。用100 µg AFM1，LPBEJ01（2×10 ⁹  CFU / ml〜2 mg / kg pc）和AFM1和LPBEJ01的混合物在3周内口服治疗LPBEJ01对小鼠AFM1免疫生物学效应的预防作用。

结果

发现体外LPBEJ01能够在24小时后吸收液体培养基中98％的AFM1（100 µg / ml），并且在固态发酵后24小时后可以去除95％以上的AFM1。用AFM1处理的动物的体重低于对照组。在用AFM1处理的小鼠中，脾脏单核细胞（SMCs）的体内促有丝分裂反应更高。用AFM1处理的小鼠的SMC产生较低水平的IL-2，较高水平的IL-4，并且对IL-10的产生没有影响。用AFM1处理的小鼠腹膜巨噬细胞释放的H ₂ O ₂较少，而口服AFM1和LPBEJ01的混合物暴露的小鼠腹膜巨噬细胞的水平更高。

结论

LPBEJ01是安全的，没有任何毒性迹象。在遭受该问题的国家中，它可以用作食品链中AFM1排毒污染的添加剂。