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Recent Progress of Glutathione (GSH) Specific Fluorescent Probes: Molecular Design, Photophysical Property, Recognition Mechanism and Bioimaging
Critical Reviews in Analytical Chemistry ( IF 4.2 ) Pub Date : 2020-09-17 , DOI: 10.1080/10408347.2020.1819193
Dugang Chen 1 , Yangzhen Feng 1
Affiliation  

Abstract

The selective detection of glutathione (GSH) in vitro and in vivo has attracted great attentions, credited to its important role in life activities and association with a series of diseases. Among all kinds of analytical techniques, the fluorescent probe for GSH detection become prevalent recently because of its ease of operation, high temporal-spatial resolution, visualization and noninvasiveness, etc. The special structural features of GSH, such as the nucleophilicity of sulfhydryl group, the concerted reaction ability of amino group, the negative charged nature, the latent hydrogen bonding ability along with its flexible molecular chain, are all potent factors to be employed to design the specific fluorescent probe for GSH and discriminate it from other bio-species including its analogues cysteine (Cys) and homocysteine (Hcy). This paper reviewed the studies in the last 3 years and was organized based on the reaction mechanism of each probe. According to the reactivity of GSH, various recognition mechanisms including Michael addition, nucleophilic aromatic substitution, ordinary nucleophilic substitution, multi-site reaction, and other unique reactions have been utilized to construct the GSH specific fluorescent probes, and the molecular design strategy, photophysical property, recognition mechanism, and bioimaging application of each reported probe were all discussed here systematically. Great progress has been made in this area, and we believe the analyses and summarization of these excellent studies would provide valuable message and inspiration to researchers to advance the research toward clinic applications.



中文翻译:

谷胱甘肽(GSH)特异性荧光探针的最新进展:分子设计、光物理性质、识别机制和生物成像

摘要

体外体内谷胱甘肽(GSH)的选择性检测因其在生命活动中的重要作用以及与一系列疾病的关联而引起了人们的广泛关注。在各种分析技术中,用于GSH检测的荧光探针由于其操作方便、时空分辨率高、可视化和无创等优点,成为近来流行的一种方法。氨基的协同反应能力、带负电荷的性质、潜在的氢键结合能力及其灵活的分子链,都是设计谷胱甘肽特异性荧光探针并将其与其他生物物种区分开来的有力因素,包括其类似物半胱氨酸(Cys)和同型半胱氨酸(Hcy)。本文回顾了近3年的研究,并根据各探针的反应机理进行整理。根据GSH的反应活性,利用迈克尔加成、亲核芳香取代、普通亲核取代、多位点反应等独特反应的多种识别机制构建了GSH特异性荧光探针,以及分子设计策略、光物理性质等。在这里系统地讨论了每个报告的探针的识别机制和生物成像应用。在这方面已经取得了很大进展,我们相信对这些优秀研究的分析和总结将为研究人员推进临床应用研究提供有价值的信息和启发。根据GSH的反应活性,利用迈克尔加成、亲核芳香取代、普通亲核取代、多位点反应等独特反应的多种识别机制构建了GSH特异性荧光探针,以及分子设计策略、光物理性质等。在这里系统地讨论了每个报告的探针的识别机制和生物成像应用。在这方面已经取得了很大进展,我们相信对这些优秀研究的分析和总结将为研究人员推进临床应用研究提供有价值的信息和启发。根据GSH的反应活性,利用迈克尔加成、亲核芳香取代、普通亲核取代、多位点反应等独特反应的多种识别机制构建了GSH特异性荧光探针,以及分子设计策略、光物理性质等。在这里系统地讨论了每个报告的探针的识别机制和生物成像应用。在这方面已经取得了很大进展,我们相信对这些优秀研究的分析和总结将为研究人员推进临床应用研究提供有价值的信息和启发。并利用其他独特的反应构建了GSH特异性荧光探针,本文系统地讨论了每种报道的探针的分子设计策略、光物理性质、识别机制和生物成像应用。在这方面已经取得了很大进展,我们相信对这些优秀研究的分析和总结将为研究人员推进临床应用研究提供有价值的信息和启发。并利用其他独特的反应构建了GSH特异性荧光探针,本文系统地讨论了每种报道的探针的分子设计策略、光物理性质、识别机制和生物成像应用。在这方面已经取得了很大进展,我们相信对这些优秀研究的分析和总结将为研究人员推进临床应用研究提供有价值的信息和启发。

更新日期:2020-09-17
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