Southern green stink bugs (Nezara viridula L.) are one of the major pests in many soybean producing areas. They cause a decrease in yield and affect seed quality by reducing viability and vigor. Alterations have been reported in the oxidative response and in the secondary metabolites in different plant species due to insect damage. However, there is little information available on soybean-stink bug interactions. In this study we compare the response of undamaged and damaged seeds by Nezara viridula in two soybean cultivars, IAC-100 (resistant) and Davis (susceptible), grown under greenhouse conditions. Pod hardness, H2O2 generation, enzyme activities in guaiacol peroxidase (GPOX), catalase (CAT) and superoxide dismutase (SOD) as well as lipoxygenase expression and isoflavonoid production were quantified. Our results showed a greater resistance of IAC-100 to pod penetration, a decrease in peroxide content after stink bug attack, and higher GPOX, CAT and SOD activities in seeds due to the genotype and to the genotype-interaction with the herbivory treatment. Induction of LOX expression in both cultivars and higher production of isoflavonoids in IAC-100 were also detected. It was then concluded that the herbivory stink bug induces pathways related to oxidative stress and to the secondary metabolites in developing seeds of soybean and that differences between cultivars hold promise for a plant breeding program.

中文翻译：

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活性氧和异黄酮在大豆抗南方绿臭虫侵袭中的作用

南方绿臭虫（Nezara viridula L.）是许多大豆产区的主要害虫之一。它们通过降低活力和活力导致产量下降并影响种子质量。据报道，由于昆虫损害，不同植物物种的氧化反应和次生代谢物发生了变化。然而，关于大豆与臭虫相互作用的信息很少。在这项研究中，我们比较了 Nezara viridula 在温室条件下生长的两种大豆品种 IAC-100（抗性）和 Davis（易感性）中未受损和受损种子的反应。豆荚硬度、H2O2 生成、愈创木酚过氧化物酶 (GPOX)、过氧化氢酶 (CAT) 和超氧化物歧化酶 (SOD) 中的酶活性以及脂氧合酶表达和异黄酮产量都被量化。我们的结果显示 IAC-100 对豆荚渗透的抵抗力更强，在臭虫攻击后过氧化物含量降低，并且由于基因型和基因型与食草处理的相互作用，种子中的 GPOX、CAT 和 SOD 活性更高。还检测到两个栽培品种中 LOX 表达的诱导和 IAC-100 中异黄酮的更高产量。然后得出的结论是，食草性臭虫在大豆种子发育过程中诱导与氧化应激和次生代谢产物相关的途径，并且品种之间的差异为植物育种计划提供了希望。还检测到两个栽培品种中 LOX 表达的诱导和 IAC-100 中异黄酮的更高产量。然后得出的结论是，食草性臭虫在大豆种子发育过程中诱导了与氧化应激和次生代谢物相关的途径，并且品种之间的差异为植物育种计划提供了希望。还检测到两个栽培品种中 LOX 表达的诱导和 IAC-100 中异黄酮的更高产量。然后得出的结论是，食草性臭虫在大豆种子发育过程中诱导了与氧化应激和次生代谢物相关的途径，并且品种之间的差异为植物育种计划提供了希望。