In this study, the transcriptional profiling of Penicillium digitatum after C₁₂O₃TR treatment was analyzed by RNA-Seq technology. A total of 2562 and 667 genes in P. digitatum were differentially expressed after 2 and 12 h treatment, respectively. These genes were respectively mapped to 91 and 79 KEGG pathways. The expression patterns of differentially expressed genes (DEGs) at 2 and 12 h were similar, mainly were the metabolic processes in cell wall, cell membrane, genetic information and energy. Particularly, the main metabolic process which was affected by C₁₂O₃TR stress for 2 and 12 h was cell integrity, including cell wall and cell membrane. The changes of chitin in cell wall was observed by Calcofluor White (CFW) staining assay. The weaker blue fluorescence in the cell wall septa, the decrease of β-1, 3-glucan synthase activity and the increase of chitinase and AKP activity showed that C₁₂O₃TR could damage the cell wall integrity. In conclusion, these results suggested that C₁₂O₃TR could inhibit the growth of P. digitatum through various mechanisms at transcriptional level, and could influence the cell wall permeability and integrity.

在这项研究中， 指状青霉通过RNA-Seq技术分析了C ₁₂ O ₃ TR处理后的结果。共有2562和667个基因洋地黄分别在2和12 h处理后差异表达。这些基因分别定位于91和79个KEGG途径。差异表达基因（DEGs）在第2和12 h的表达方式相似，主要是细胞壁，细胞膜的代谢过程，遗传信息和能量。特别地，受C ₁₂ O ₃ TR应力影响2和12 h的主要代谢过程是细胞完整性，包括细胞壁和细胞膜。用钙荧光白（CFW）染色法观察细胞壁中几丁质的变化。细胞壁中的蓝色荧光减弱，β-1、3-葡聚糖合酶活性降低，几丁质酶和AKP活性升高，表明C ₁₂ O ₃TR可能会破坏细胞壁的完整性。总之，这些结果表明，C ₁₂ O ₃ TR可以抑制藻类的生长。洋地黄 通过转录水平的各种机制，并可能影响细胞壁的通透性和完整性。