Improving probiotic spore yield using rice straw hydrolysate,Letters in Applied Microbiology

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Improving probiotic spore yield using rice straw hydrolysate
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-11-08 , DOI: 10.1111/lam.13387
L Yin _{1,

2} , M X Chen ₁ , T H Zeng ₁ , X M Liu ₃ , F Zhu ₄ , R Q Huang _{1,

2}

Affiliation

Spore-forming Bacillus sp. has been extensively studied for their probiotic properties. In this study, an acid-treated rice straw hydrolysate was used as carbon source to produce the spores of Bacillus coagulans. The results showed that this hydrolysate significantly improved the spore yield compared with other carbon sources such as glucose et al. Three significant medium components including rice straw hydrolysate, MnSO4 and yeast extract were screened by Plackett-Burman design (PBD). These significant variables were further optimized by response surface methodology (RSM). The optimal values of the medium components were rice straw hydolysate of 27% (v/v), MnSO4 of 0.78 g l-1 and yeast extract of 1.2 g l-1 . The optimized medium and RSM model for spore production were validated in a 5L bioreactor. Overall, this sporulation medium containing acid-treated rice straw hydrolysate has a potential to be used in the production of B. coagulans spores.

中文翻译：

利用稻草水解物提高益生菌孢子产量

产芽孢杆菌。已对其益生菌特性进行了广泛的研究。在这项研究中，酸处理的稻草水解物被用作碳源来生产凝结芽孢杆菌的孢子。结果表明，与葡萄糖等其他碳源相比，该水解产物显着提高了孢子产量。通过 Plackett-Burman 设计 (PBD) 筛选出三种重要的培养基成分，包括稻草水解物、MnSO4 和酵母提取物。这些重要变量通过响应面方法 (RSM) 进一步优化。培养基组分的最佳值是27% (v/v) 的稻草水解物、0.78 g l-1 的MnSO4 和1.2 g l-1 的酵母提取物。用于孢子生产的优化培养基和 RSM 模型在 5L 生物反应器中进行了验证。全面的，

更新日期：2020-11-08

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