For the sustainable farming of tilapia, proper maintenance of their health and adequate treatment for infections at appropriate time are inevitable. The indiscriminate use of antibiotics in aquaculture, as a part of treatment and as growth promoters, accelerates antimicrobial resistance (AMR) among the fish pathogens. In the present study, we have isolated diverse aeromonads from Nile tilapia and studied their antibiogram and plasmid profiling. Aeromonas hydrophila, Aeromonas veronii, Aeromonas sobria, Aeromonas dhakensis, Aeromonas caviae, Aeromonas jandaei and Aeromonas aquatica were isolated from infected tilapia (n = 150), and their Shannon wiener diversity index was calculated as 1.926. A. veronii was found to be the most multiple antibiotic‐resistant pathogen with the MAR index of 0.46, and A. aquatica was noticed as the least resistant isolate. The minimum inhibitory concentration of resistant antibiotics was shown as >256 mcg/ml for most of the isolates. The virulent genes such as aerolysin and hemolysin were identified in all the isolates except A. aquatica. The detection of class 1 integrons, plasmid profiling and plasmid curing studies confirmed that AMR exhibited by most of the Aeromonas species is of plasmid mediated. This challenges the risk of wide spread of AMR among the pathogens and subsequent treatment of the infection.

中文翻译：

---

患病的尼罗罗非鱼（Oreochromis niloticus）的运动型气单胞菌中的质粒介导的抗药性

为了罗非鱼的可持续养殖，不可避免的是要适当维护其健康并在适当的时候对感染进行适当的治疗。作为治疗的一部分和作为生长促进剂，在水产养殖中不加选择地使用抗生素会加速鱼类病原体之间的抗药性（AMR）。在本研究中，我们从尼罗罗非鱼中分离了多种气单胞菌，并研究了它们的抗菌素谱和质粒图谱。嗜水，气单胞菌维罗纳，气单胞sobria，气单胞菌dhakensis，气单胞菌的豚鼠气，气单胞菌jandaei和嗜菜类从受感染的罗非鱼分离（Ñ = 150），其香农维纳多样性指数经计算为1.926。A.维罗纳被发现是最多个抗生素抗性病原体具有0.46的指数MAR，和A.菜类，注意到作为至少抗性分离物。对于大多数分离物，抗性抗生素的最小抑制浓度显示为> 256 mcg / ml。在除水曲霉外的所有菌株中都鉴定出了诸如溶血素和溶血素等毒力基因。对1类整联蛋白的检测，质粒谱分析和质粒固化研究证实，大多数气单胞菌物种表现出的AMR是质粒介导的。这挑战了AMR在病原体中广泛传播以及感染的后续治疗的风险。