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Comprehensive profiling of protein lysine acetylation and its overlap with lysine succinylation in the Porphyromonas gingivalis fimbriated strain ATCC 33277.
Molecular Oral Microbiology ( IF 2.8 ) Pub Date : 2020-09-16 , DOI: 10.1111/omi.12312 Jumei Zeng 1 , Leng Wu 2, 3 , Qiushi Chen 4 , Lingyun Wang 5 , Wei Qiu 2 , Xin Zheng 2 , Xiaoming Yin 4 , Jie Liu 4 , Yan Ren 4 , Yuqing Li 2
Molecular Oral Microbiology ( IF 2.8 ) Pub Date : 2020-09-16 , DOI: 10.1111/omi.12312 Jumei Zeng 1 , Leng Wu 2, 3 , Qiushi Chen 4 , Lingyun Wang 5 , Wei Qiu 2 , Xin Zheng 2 , Xiaoming Yin 4 , Jie Liu 4 , Yan Ren 4 , Yuqing Li 2
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Porphyromonas gingivalis is a pathogen closely associated with periodontal and systemic infections. Recently, lysine acetylation (Kac) and lysine succinylation (Ksuc) have been identified in bacterial proteins with diverse biological and pathological functions. The Ksuc of P. gingivalis ATCC 33277 has been characterized in our previous work, and here, we report the systematic analysis of Kac and its crosstalk with Ksuc in this bacterium. A combination of the affinity enrichment by the acetyl‐lysine antibody with highly sensitive LC‐MS/MS was used to identify the lysine‐acetylated proteins and sites in P. gingivalis ATCC 33277. A total of 1,112 lysine‐acetylated sites matching 438 proteins were identified. These proteins involved in several cellular processes, especially those proteins related to protein biosynthesis and central metabolism had a high tendency to be lysine acetylated. Moreover, lysine sites flanked by tyrosine, phenylalanine, and histidine in the +1 position, as well as residue lysine in position +4 to +5, were the targets of Kac. Additionally, proteins involved in adhesins, gingipains, black pigmentation, and oxidative stress resistance were identified as substrates of Kac. Collectively, these results suggest Kac may play a critical role in the regulation of physiology and virulence of P. gingivalis. Furthermore, we discovered that, Ksuc and Kac were extensively overlapped in P. gingivalis ATCC 33277, especially in proteins related to ribosomes and metabolism. This study provides a significant beginning for further investigating the role of Kac and Ksuc in the pathogenicity of P. gingivalis.
中文翻译：
牙龈卟啉单胞菌菌丝菌株ATCC 33277中蛋白质赖氨酸乙酰化的全面概况分析及其与赖氨酸琥珀酰化的重叠。
牙龈卟啉单胞菌是与牙周和全身感染密切相关的病原体。最近，已经在具有多种生物学和病理学功能的细菌蛋白中鉴定出赖氨酸乙酰化（Kac）和赖氨酸琥珀酰化（Ksuc）。牙龈卟啉单胞菌ATCC 33277的Ksuc已在我们先前的工作中进行了表征，在这里，我们报告了对该细菌中Kac及其与Ksuc的串扰的系统分析。将乙酰赖氨酸抗体的亲和力富集与高度敏感的LC-MS / MS结合起来用于鉴定牙龈卟啉单胞菌中赖氨酸乙酰化的蛋白质和位点ATCC 33277.总共鉴定了1,112个与438个蛋白质匹配的赖氨酸乙酰化位点。这些蛋白质参与多个细胞过程，特别是与蛋白质生物合成和中枢代谢有关的那些蛋白质具有被赖氨酸乙酰化的高度趋势。此外，Kac的目标是在+1位上侧接酪氨酸，苯丙氨酸和组氨酸的赖氨酸位点以及在+4至+5位上的赖氨酸残基。此外，参与粘附素，牙龈蛋白酶，黑色素沉着和抗氧化应激的蛋白质被确定为Kac的底物。总的来说，这些结果表明Kac可能在牙龈卟啉单胞菌的生理和毒力的调节中起关键作用。此外，我们发现，Ksuc和Kac在牙龈卟啉单胞菌中广泛重叠。ATCC 33277，尤其是与核糖体和代谢有关的蛋白质。该研究为进一步研究Kac和Ksuc在牙龈卟啉单胞菌致病性中的作用提供了重要的起点。
更新日期：2020-09-16
中文翻译：
牙龈卟啉单胞菌菌丝菌株ATCC 33277中蛋白质赖氨酸乙酰化的全面概况分析及其与赖氨酸琥珀酰化的重叠。
牙龈卟啉单胞菌是与牙周和全身感染密切相关的病原体。最近，已经在具有多种生物学和病理学功能的细菌蛋白中鉴定出赖氨酸乙酰化（Kac）和赖氨酸琥珀酰化（Ksuc）。牙龈卟啉单胞菌ATCC 33277的Ksuc已在我们先前的工作中进行了表征，在这里，我们报告了对该细菌中Kac及其与Ksuc的串扰的系统分析。将乙酰赖氨酸抗体的亲和力富集与高度敏感的LC-MS / MS结合起来用于鉴定牙龈卟啉单胞菌中赖氨酸乙酰化的蛋白质和位点ATCC 33277.总共鉴定了1,112个与438个蛋白质匹配的赖氨酸乙酰化位点。这些蛋白质参与多个细胞过程，特别是与蛋白质生物合成和中枢代谢有关的那些蛋白质具有被赖氨酸乙酰化的高度趋势。此外，Kac的目标是在+1位上侧接酪氨酸，苯丙氨酸和组氨酸的赖氨酸位点以及在+4至+5位上的赖氨酸残基。此外，参与粘附素，牙龈蛋白酶，黑色素沉着和抗氧化应激的蛋白质被确定为Kac的底物。总的来说，这些结果表明Kac可能在牙龈卟啉单胞菌的生理和毒力的调节中起关键作用。此外，我们发现，Ksuc和Kac在牙龈卟啉单胞菌中广泛重叠。ATCC 33277，尤其是与核糖体和代谢有关的蛋白质。该研究为进一步研究Kac和Ksuc在牙龈卟啉单胞菌致病性中的作用提供了重要的起点。
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