Conducting polymer-based electrochemical biosensor for the detection of acetylthiocholine and pesticide via acetylcholinesterase,Biotechnology and Applied Biochemistry

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Conducting polymer-based electrochemical biosensor for the detection of acetylthiocholine and pesticide via acetylcholinesterase
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-09-17 , DOI: 10.1002/bab.2030
Abdurrahman Akdag ₁ , Mesut Işık _{2,

3} , Hasan Göktaş ₄

Affiliation

A voltammetric biosensor for acetylthiocholine (ATCh) and paraoxon detection was successfully developed. To achieve this goal, polypyrrole (PPy) was synthesized onto the platinum (Pt) electrode surface in 0.30 M oxalic acid solution containing 25 mM pyrrole. PPy-coated Pt (Pt/PPy) electrode surface was covered with chitosan (Chi) (Pt/PPy/Chi). The acetylcholinesterase (AChE) enzyme was immobilized on the Pt/PPy/Chi electrode surface to build a voltammetric biosensor (Pt/PPy/Chi/AChE). The storage stability of the biosensor was determined to be 72% even after 60 days. The operational stability was determined to be 94% after 20 consecutive measurements. For the biosensor, the linear range was determined to be 30–50 µM for ATCh and 0.46–1.84 nM for paraoxon. The limit of detection (LOD) was determined to be 0.45 µM for ATCh and 0.17 nM for paraoxon

中文翻译：

基于聚合物的电化学生物传感器通过乙酰胆碱酯酶检测乙酰硫代胆碱和农药

成功研制了一种用于乙酰硫代胆碱（ATCh）和对氧磷检测的伏安生物传感器。为了实现这一目标，在含有 25 mM 吡咯的 0.30 M 草酸溶液中，在铂 (Pt) 电极表面合成了聚吡咯 (PPy)。PPy 涂层 Pt (Pt/PPy) 电极表面覆盖有壳聚糖 (Chi) (Pt/PPy/Chi)。将乙酰胆碱酯酶 (AChE) 酶固定在 Pt/PPy/Chi 电极表面以构建伏安生物传感器 (Pt/PPy/Chi/AChE)。即使在 60 天后，生物传感器的储存稳定性也被确定为 72%。连续 20 次测量后确定操作稳定性为 94%。对于生物传感器，ATCh 的线性范围被确定为 30–50 µM，对氧磷的线性范围为 0.46–1.84 nM。ATCh 的检测限 (LOD) 为 0.45 µM，对氧磷为 0.17 nM

更新日期：2020-09-17

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