Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Latest Advances for the Sleeping Beauty Transposon System: 23 Years of Insomnia but Prettier than Ever: Refinement and Recent Innovations of the Sleeping Beauty Transposon System Enabling Novel, Nonviral Genetic Engineering Applications.
BioEssays ( IF 3.2 ) Pub Date : 2020-09-16 , DOI: 10.1002/bies.202000136 Maximilian Amberger 1 , Zoltán Ivics 1
BioEssays ( IF 3.2 ) Pub Date : 2020-09-16 , DOI: 10.1002/bies.202000136 Maximilian Amberger 1 , Zoltán Ivics 1
Affiliation
|
The Sleeping Beauty transposon system is a nonviral DNA transfer tool capable of efficiently mediating transposition‐based, stable integration of DNA sequences of choice into eukaryotic genomes. Continuous refinements of the system, including the emergence of hyperactive transposase mutants and novel approaches in vectorology, greatly improve upon transposition efficiency rivaling viral‐vector‐based methods for stable gene insertion. Current developments, such as reversible transgenesis and proof‐of‐concept RNA‐guided transposition, further expand on possible applications in the future. In addition, innate advantages such as lack of preferential integration into genes reduce insertional mutagenesis‐related safety concerns while comparably low manufacturing costs enable widespread implementation. Accordingly, the system is recognized as a powerful and versatile tool for genetic engineering and is playing a central role in an ever‐expanding number of gene and cell therapy clinical trials with the potential to become a key technology to meet the growing demand for advanced therapy medicinal products.
中文翻译：
睡美人转座子系统的最新进展：23 年的失眠症，但比以往任何时候都更漂亮：睡美人转座子系统的改进和最新创新使新的非病毒基因工程应用成为可能。
睡美人_转座子系统是一种非病毒 DNA 转移工具，能够有效地介导基于转座的、稳定地将所选 DNA 序列整合到真核基因组中。该系统的不断改进，包括高活性转座酶突变体的出现和载体学中的新方法，大大提高了转座效率，可与基于病毒载体的稳定基因插入方法相媲美。当前的发展，例如可逆转基因和概念验证 RNA 引导的转座，进一步扩展了未来可能的应用。此外，先天优势，例如缺乏优先整合到基因中，减少了与插入诱变相关的安全问题，而相对较低的制造成本使广泛实施成为可能。因此，
更新日期：2020-10-22
中文翻译：
睡美人转座子系统的最新进展：23 年的失眠症，但比以往任何时候都更漂亮：睡美人转座子系统的改进和最新创新使新的非病毒基因工程应用成为可能。
睡美人_转座子系统是一种非病毒 DNA 转移工具，能够有效地介导基于转座的、稳定地将所选 DNA 序列整合到真核基因组中。该系统的不断改进，包括高活性转座酶突变体的出现和载体学中的新方法，大大提高了转座效率，可与基于病毒载体的稳定基因插入方法相媲美。当前的发展，例如可逆转基因和概念验证 RNA 引导的转座，进一步扩展了未来可能的应用。此外，先天优势，例如缺乏优先整合到基因中，减少了与插入诱变相关的安全问题，而相对较低的制造成本使广泛实施成为可能。因此，
京公网安备 11010802027423号