Dynamic measurements of steroid hormones in vivo are critical, but steroid sensing is currently limited by the availability of specific molecular recognition elements due to the chemical similarity of these hormones. In this work, a new, self‐templating synthetic approach is applied using corona phase molecular recognition (CoPhMoRe) targeting the steroid family of molecules to produce near infrared fluorescent, implantable sensors. A key limitation of CoPhMoRe has been its reliance on library generation for sensor screening. This problem is addressed with a self‐templating strategy of polymer design, using the examples of progesterone and cortisol sensing based on a styrene and acrylic acid copolymer library augmented with an acrylated steroid. The pendant steroid attached to the corona backbone is shown to self‐template the phase, providing a unique CoPhMoRE design strategy with high efficacy. The resulting sensors exhibit excellent stability and reversibility upon repeated analyte cycling. It is shown that molecular recognition using such constructs is viable even in vivo after sensor implantation into a murine model by employing a poly (ethylene glycol) diacrylate (PEGDA) hydrogel and porous cellulose interface to limit nonspecific absorption. The results demonstrate that CoPhMoRe templating is sufficiently robust to enable a new class of continuous, in vivo biosensors.

中文翻译：

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使用自定型电晕相分子识别技术的人体类固醇激素体内植入式纳米传感器。

体内类固醇激素的动态测量至关重要，但由于这些激素的化学相似性，目前类固醇感测受到特定分子识别元件可用性的限制。在这项工作中，采用了一种新的，自我模板化的合成方法，该方法使用了针对类固醇分子家族的电晕相分子识别（CoPhMoRe），以产生近红外荧光的可植入传感器。CoPhMoRe的一个关键局限性在于它对用于传感器筛选的库的依赖。使用基于模板的自我模板化策略来解决该问题，该示例基于基于丙烯酸酯类固醇增强的苯乙烯和丙烯酸共聚物库的孕酮和皮质醇检测实例。显示出附在电晕主链上的垂体类固醇可以自我模板化该相，提供高效的独特CoPhMoRE设计策略。重复的分析物循环后，所得传感器表现出出色的稳定性和可逆性。通过使用聚（乙二醇）二丙烯酸酯（PEGDA）水凝胶和多孔纤维素界面来限制非特异性吸收，已表明使用这种构建体的分子识别甚至在传感器植入鼠模型后的体内也是可行的。结果表明，CoPhMoRe模板具有足够的鲁棒性，可以实现一类新型的连续体内生物传感器。通过使用聚（乙二醇）二丙烯酸酯（PEGDA）水凝胶和多孔纤维素界面来限制非特异性吸收，已表明使用这种构建体的分子识别甚至在传感器植入鼠模型后的体内也是可行的。结果表明，CoPhMoRe模板具有足够的鲁棒性，可以实现一类新型的连续体内生物传感器。通过使用聚（乙二醇）二丙烯酸酯（PEGDA）水凝胶和多孔纤维素界面来限制非特异性吸收，已表明使用这种构建体的分子识别甚至在传感器植入鼠模型后的体内也是可行的。结果表明，CoPhMoRe模板具有足够的鲁棒性，可以实现一类新型的连续体内生物传感器。