The pervasive spread of infectious diseases and pandemics, such as the 2019 coronavirus disease (COVID-19), are becoming increasingly serious and urgent threats to human health. Preventing the spread of such diseases prioritizes the development of sensing devices that can rapidly, selectively, and reliably detect pathogens at minimal cost. Paper-based analytical devices (PADs) are promising tools that satisfy those criteria. Numerous paper-based biosensors have been established that rival conventional pathogen detection methods. Among them, colorimetric strategies are promising since results can be interpreted by eye, and are simple to operate, which is advantageous for point-of-care testing (POCT). Particularly, the application of nanomaterials on paper-based biosensors has become important as these materials are capable of converting signals from pathogens through unique mechanisms to yield an amplified colorimetric readout. To highlight the research progress on using nanomaterials in colorimetric paper-based biosensor for pathogen detection, we discuss the sensing mechanisms of how they work, structural and analytical characteristics of the devices, and representative recent applications. Current challenges and future directions of using PADs and nanomaterial-mediated strategies are also discussed.

传染病和流行病的普遍传播，例如 2019 年冠状病毒病 (COVID-19)，对人类健康构成日益严重和紧迫的威胁。预防此类疾病的传播优先考虑开发能够以最低成本快速、选择性和可靠地检测病原体的传感设备。纸质分析设备 (PAD) 是满足这些标准的有前途的工具。已经建立了许多与传统病原体检测方法相媲美的纸质生物传感器。其中，比色策略很有前景，因为结果可以通过肉眼解释，并且操作简单，有利于即时检测 (POCT)。特别，纳米材料在纸基生物传感器上的应用变得非常重要，因为这些材料能够通过独特的机制转换来自病原体的信号，从而产生放大的比色读数。为了突出在比色纸基生物传感器中使用纳米材料进行病原体检测的研究进展，我们讨论了它们如何工作的传感机制、设备的结构和分析特性以及最近的代表性应用。还讨论了使用 PAD 和纳米材料介导策略的当前挑战和未来方向。设备的结构和分析特性，以及最近的代表性应用。还讨论了使用 PAD 和纳米材料介导策略的当前挑战和未来方向。设备的结构和分析特性，以及最近的代表性应用。还讨论了使用 PAD 和纳米材料介导策略的当前挑战和未来方向。