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Generation of two human induced pluripotent stem cell lines derived from myoblasts (MDCi014-A) and from peripheral blood mononuclear cells (MDCi014-B) from the same donor
Stem Cell Research ( IF 0.8 ) Pub Date : 2020-09-17 , DOI: 10.1016/j.scr.2020.101998
Eric Metzler 1 , Narasimha Telugu 2 , Sebastian Diecke 3 , Simone Spuler 4 , Helena Escobar 1
Affiliation  

We describe the generation and characterization of two human induced pluripotent stem cell (hiPSCs) lines reprogrammed from myoblasts and from peripheral blood mononuclear cells (PBMCs) from the same donor. The donor was free of neuromuscular disorders, male and 18 years of age. For reprogramming we used Sendai-virus delivery of the four Yamanaka factors. The pluripotent identity of the hiPSC lines was confirmed by the expression of pluripotency markers and their capacity to differentiate into all three germ layers. These hiPSCs constitute a tool to study tissue of origin specific differences in the identity of hiPSCs.



中文翻译:

从同一供体的成肌细胞(MDCi014-A)和外周血单个核细胞(MDCi014-B)衍生出两种人类诱导的多能干细胞系

我们描述了从成肌细胞和来自同一供体的外周血单核细胞(PBMC)重新编程的两个人诱导多能干细胞(hiPSCs)系的产生和表征。供体没有男性和18岁的神经肌肉疾病。为了进行重新编程,我们使用了仙台病毒传递的四个山中因子。通过多能性标志物的表达及其分化为所有三个胚层的能力,证实了hiPSC系的多能性身份。这些hiPSC构成了一种工具,用于研究hiPSC身份方面的起源特定组织差异。

更新日期:2020-09-23
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