We presented herien the rational design, synthesis, and photophysical property studies of the lysosome-targeted fluorescence FA probe NP-Lyso, an isopropyl group modified ortho-diaminonaphthalimide derivative. After the reaction of FA and ortho-phenylenediamine modified with the isopropyl group in NP-Lyso, the probe exhibited favorable features such as a large fluorescence enhancement, specific selectivity and high sensitivity for the detection of FA. More importantly, NP-Lyso could be used to detect and image endogenous FA in lysosomes. In light of these prominent properties, we envision that NP-Lyso will be an efficient optical imaging approach for investigating the biofunctions of FA in living systems.

我们介绍了herien的溶酶体靶向荧光FA探针NP-Lyso（一种异丙基修饰的邻二氨基萘二酰亚胺衍生物）的合理设计，合成和光物理性质研究。FA与NP-Lyso中经异丙基修饰的邻苯二胺反应后，探针具有良好的荧光增强，比选择性高，灵敏度高等特点。更重要的是，NP-Lyso可用于检测溶酶体中的内源性FA并对其成像。鉴于这些突出的特性，我们设想NP-Lyso 将是一种用于研究FA在生命系统中的生物功能的高效光学成像方法。