Aqueous two-phase extraction (ATPE) is an emerging technique that integrates separation, concentration, and partial purification of target compounds from natural resources. One potential issue which may prevent industrial application of ATPE, is the large consumption of phase-forming components such as alcohol, polymers, salt, and sugar. On top of that, post-handling and disposal of these components will increase associated costs. This research evaluated the effects of recycling ATPE components on the extraction of bioactive compounds from haskap (Lonicera caerulea) leaves. In this work, the ATPE systems were combinations of ammonium sulphate/ethanol, sodium dihydrogen phosphate/ethanol, glucose/1-propanol, and maltose/1-propanol. Total phenolic content (TPC), flavonoids, and chlorogenic acid were assessed in terms of their partitioning behavior, extraction efficiencies, and yields. The results showed that with two recycling stages, the extraction performance for all four ATPE systems was maintained. The average TPC extraction efficiencies across the first extraction stage and two recycling stages were 91.4% for ammonium sulphate/ethanol, 99.6% for sodium dihydrogen phosphate/ethanol, 85.1% for glucose/1-propanol, and 85.0% for maltose/1-propanol systems. This suggests that recycling could make ATPE a more sustainable technique in recovering high-value compounds from natural resources.

水两相萃取（ATPE）是一种新兴技术，它结合了从天然资源中分离，浓缩和部分纯化目标化合物的功能。可能阻止ATPE工业应用的一个潜在问题是大量消耗相形成成分，例如醇，聚合物，盐和糖。最重要的是，这些组件的后期处理和处置将增加相关成本。这项研究评估了回收ATPE成分对从haskap（Lonicera caerulea）中提取生物活性化合物的影响） 树叶。在这项工作中，ATPE系统是硫酸铵/乙醇，磷酸二氢钠/乙醇，葡萄糖/ 1-丙醇和麦芽糖/ 1-丙醇的组合。根据总酚含量（TPC），类黄酮和绿原酸的分配行为，提取效率和产率进行了评估。结果表明，在两个回收阶段，所有四个ATPE系统的萃取性能均得以保持。在第一个萃取阶段和两个回收阶段中，TPC的平均萃取效率分别为：硫酸铵/乙醇为91.4％，磷酸二氢钠/乙醇为99.6％，葡萄糖/ 1-丙醇为85.1％，麦芽糖/ 1-丙醇为85.0％。系统。这表明，回收利用可使ATPE成为从自然资源中回收高价值化合物的更具可持续性的技术。