Pigments, such as chlorophylls and carotenoids, have important applications in various fields, such as colorants in food, cosmetic or textile industries and in biomedical applications. Both pigments have an important role in the photosynthetic process and can be found in the marine green macroalgae genus Ulva. In this work, an integrated downstream process was developed to extract chlorophylls and carotenoids from those macroalgae. The solid-liquid extraction was optimized. For that, several conditions were tested, namely the use of different mechanical processes (maceration, microwave- or ultrasound- assisted extraction), type of solvent, number of consecutive extractions, solid-liquid ratio, and the design of the extraction process using a mechanical treatment. Using the extract obtained, a liquid-liquid extraction system composed of ethanol, hexane, and water was then studied. Different mixture points within the biphasic region were tested in terms of their ability to selectively separate chlorophylls and xanthophylls to opposite phases in a single step. The optimization and implementation of a simple, fast and efficient downstream process to separate both classes of pigments such as chlorophylls and xanthophylls from green macroalgae is reported. It could be applied to fractionate other extracts with similar compositions obtained from other natural sources.

诸如叶绿素和类胡萝卜素的颜料在各个领域具有重要的应用，例如食品，化妆品或纺织工业中的着色剂以及生物医学应用。两种色素在光合作用过程中都具有重要作用，并且可以在海洋绿色大型藻类Ulva中找到。在这项工作中，开发了一个集成的下游过程，以从那些大型藻类中提取叶绿素和类胡萝卜素。优化了固液萃取。为此，测试了几种条件，即使用不同的机械过程（浸渍，微波或超声辅助萃取），溶剂类型，连续萃取次数，固液比以及使用萃取液设计萃取过程。机械处理。使用获得的提取物，然后研究了由乙醇，己烷和水组成的液-液提取系统。测试了双相区域内不同的混合点，使其能够在单个步骤中将叶绿素和叶黄素选择性分离为相反相。优化和实现简单，据报道，从绿色大型藻类中分离出两类色素（例如叶绿素和叶黄素）的快速有效的下游工艺。它可用于分馏具有与其他天然来源相似组成的其他提取物。