This study was aimed to analyze the anti-cancer activity of silver nanoparticles (AgNPs) synthesized using aqueous plant extracts from the rhizome of Curcuma longa and Zingiber officinale. Synergistic aqueous extract of rhizome of C. longa and Z. officinale was used to green synthesis of AgNPs. Characterization of AgNPs was performed using UV–visible spectroscopy, FTIR, X-ray diffraction, TEM, and SEM analyses. Anti-cancer activity of AgNPs against human colon carcinoma (HT-29) cells was tested using MTT assay. UV–Visible spectroscopy analysis indicated the surface plasmon resonance (SPR) sharp peak at 350–430 nm wavelength that corresponds to the production of AgNPs. FTIR analysis reveals that existence of carboxyl (CO) and amine (NH) functional groups in the AgNPs. The X-ray diffraction analysis confirms four spectral peaks at 111, 200, 220, and 311. SEM analysis showed that AgNPs are in a spherical shape with a size of 42–61 nm and TEM analysis showed particle size are ranged between 20–51 nm. Anti-cancer study reveals that AgNPs had shown cytotoxicity against HT-29 cells at the concentrations ranged from 25 to 500 μg/mL and IC₅₀ at 150.8 µg/mL. This study concludes that AgNPs synthesized using rhizome of Z. officinale and C. longa possesses potential anti-cancer activity.

本研究旨在分析使用姜黄和姜根茎的水性植物提取物合成的银纳米粒子 (AgNPs) 的抗癌活性。将龙舌草和铁皮的根茎协同水提物用于AgNPs的绿色合成。使用紫外-可见光谱、FTIR、X 射线衍射、TEM 和 SEM 分析对 AgNPs 进行表征。使用 MTT 法测试 AgNPs 对人结肠癌 (HT-29) 细胞的抗癌活性。紫外-可见光谱分析表明表面等离子共振 (SPR) 在 350-430 nm 波长处有尖峰，对应于 AgNPs 的产生。FTIR 分析表明存在羧基 ( CAgNPs 中的O) 和胺 ( NH) 官能团。X 射线衍射分析证实了 111、200、220 和 311 处的四个光谱峰。SEM 分析表明，AgNPs 呈球形，尺寸为 42-61 nm，TEM 分析显示粒径在 20-51 之间纳米。抗癌研究表明，AgNPs 在 25 至 500 μg/mL 的浓度范围内显示出对 HT-29 细胞的细胞毒性，IC ₅₀在 150.8 μg/mL 的范围内。本研究得出结论，使用Z. officinale 和 C. longa的根茎合成的 AgNPs具有潜在的抗癌活性。