Staphylococcus aureus (S. aureus), which has developed multidrug resistance, leads to many healthcare-associated infections resulting in significant medical and economic losses. Therefore, the development of new efficient strategies to deal with these bacteria has been gaining importance. Lysostaphin is a peptidoglycan hydrolase that has considerable potential as a bacteriocin. However, there have been few reported optimization and scale-up studies of the lysostaphin bioproduction process. Our preliminary results have revealed that the composition of auto-induction media at 30 °C increases the produced lysostaphin around 10-fold in shake flasks. In this study, achieving higher yields for recombinant lysostaphin in E. coli at a laboratory scale has been the aim, through the use of auto-induction media.

Optimized medium composition and fermentation parameters were transferred to a laboratory-scale bioreactor. The tested conditions improved protein yields up to 184 mg/L in a 3 L stirred bioreactor and the productivity was improved 2-fold in comparison to previously published reports. Furthermore, this study also showed that lysostaphin is an effective bacteriocin on both commercially available and isolated S. aureus strains. These results will contribute to future larger-scale production of lysostaphin via the proposed fermentation conditions.

金黄色葡萄球菌（S. aureus）已发展成多药耐药性，导致许多与医疗保健有关的感染，造成重大的医疗和经济损失。因此，开发用于处理这些细菌的新的有效策略的重要性日益增加。溶葡萄球菌素是肽聚糖水解酶，具有作为细菌素的巨大潜力。然而，鲜有关于溶葡萄球菌素生物生产过程的优化和放大研究的报道。我们的初步结果表明，在摇瓶中，在30°C时自动诱导培养基的成分会使产生的溶葡萄球菌素增加约10倍。在这项研究中，在大肠杆菌中获得更高的重组溶葡萄球菌素产量 通过使用自动感应介质，已成为实验室规模的目标。

将优化的培养基组成和发酵参数转移至实验室规模的生物反应器。在3 L搅拌的生物反应器中，经过测试的条件将蛋白质产量提高至184 mg / L，与以前发表的报告相比，生产率提高了2倍。此外，这项研究还表明，溶葡萄球菌素对市售和分离的金黄色葡萄球菌菌株均是有效的细菌素。这些结果将有助于未来通过提议的发酵条件大规模生产溶葡萄球菌素。