This study aims to investigate the effects of intrauterine perfusion of granulocyte colony-stimulating factor (G-CSF) on a thin-endometrium rat model. Twenty rats in two groups of 10 were used. Group I was perfused with normal saline (NS) in the right uterine horn and 95% ethanol in the left one. Group II was bilaterally perfused with 95% ethanol into the uterine horns. After three estrous cycles, Group II was perfused with NS in the right uterine horn and G-CSF (30 μg/kg) in the left one. Hematoxylin–eosin (HE) and immunohistochemistry (IHC) staining were used to detect changes in endometrial thickness and expression of cytokeratin 19 (CK19) and vimentin (Vim). The relative expression levels of vascular endothelial growth factor () and leukemia inhibitory factor () were also tested via reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) and Western-blot analyses. G-CSF treatment significantly increased the thickness of the endometrium in the 95% ethanol-induced thin-endometrium rat model. The expression levels of endometrial glandular epithelial cell marker for CK19 and stromal cell marker Vim were augmented in the G-CSF-treated group compared with the control group. Moreover, G-CSF treatment stimulated the expression of VEGF and LIF in the 95% ethanol-induced thin-endometrium rat model. G-CSF intrauterine perfusion improved endometrial receptivity in the thin-endometrium rat model by stimulating endometrial proliferation and angiogenesis.

中文翻译：

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宫内灌注粒细胞集落刺激因子对薄子宫内膜大鼠模型的治疗作用及机制


本研究旨在探讨宫内灌注粒细胞集落刺激因子（G-CSF）对薄子宫内膜大鼠模型的影响。使用 20 只大鼠，分为两组，每组 10 只。第一组在右侧子宫角灌注生理盐水（NS），在左侧子宫角灌注95％乙醇。 II组双侧子宫角灌注95%乙醇。三个动情周期后，第二组在右侧子宫角灌注 NS，在左侧子宫角灌注 G-CSF (30 µg/kg)。采用苏木精-伊红 (HE) 和免疫组织化学 (IHC) 染色检测子宫内膜厚度以及细胞角蛋白 19 (CK19) 和波形蛋白 (Vim) 表达的变化。还通过逆转录定量实时聚合酶链反应（RT-qPCR）和蛋白质印迹分析测试了血管内皮生长因子（）和白血病抑制因子（）的相对表达水平。 G-CSF治疗显着增加了95%乙醇诱导的薄子宫内膜大鼠模型中的子宫内膜厚度。与对照组相比，G-CSF治疗组子宫内膜腺上皮细胞标记物CK19和基质细胞标记物Vim的表达水平有所增加。此外，G-CSF治疗刺激了95%乙醇诱导的薄子宫内膜大鼠模型中VEGF和LIF的表达。 G-CSF宫内灌注通过刺激子宫内膜增殖和血管生成，改善薄子宫内膜大鼠模型的子宫内膜容受性。