Fast saccharide mapping method for quality consistency evaluation of commercial xylooligosaccharides collected in China,Journal of Pharmaceutical Analysis

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Fast saccharide mapping method for quality consistency evaluation of commercial xylooligosaccharides collected in China
Journal of Pharmaceutical Analysis ( IF 6.1 ) Pub Date : 2020-09-17 , DOI: 10.1016/j.jpha.2020.08.013
Yong Deng ₁ , Cunwu Chen ₂ , Lingxiao Chen ₁ , Bangxing Han ₂ , Shaoping Li ₁ , Jing Zhao ₁

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Due to the extensive use of xylooligosaccharides (XOS) as functional food ingredients, many inferior goods and even adulterants are generally found in the market, which may pose a health hazard to certain populations. Chromatography method such as high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) is traditionally applied for the quality analysis of XOS. However, it is time consuming due to the prolonged separation and pre- or post- derivatization procedure. In this study, a fast saccharide mapping method based on matrix-assisted laser desorption/time-of-flight mass spectrometry (MALDI-TOF-MS) was developed for the quality consistency analysis of 22 batches of XOS collected from different manufacturers in China. The time needed for saccharides analysis using MALDI-MS was less than 30 min for one plate, at least 6 times faster than that by the traditional HPTLC chromatography method. In addition, MALDI-MS possessed higher resolution for XOS with DP4-DP7 based on the difference of /, which is hardly separated using HPTLC. The results showed that XOS were present only in samples XY01-XY11, samples XY12-XY14 only consisted of hex oligosaccharides, and samples XY15-XY22 were free of oligosaccharides. These indicate that the quality consistency of XOS products in the China market was poor, which should be carefully investigated.

更新日期：2020-09-17

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