Coix seed oil (CSO) is a kind of fatty oil extracted from coix seed, which has broad anticancer effects. Using CSO as the oil phase in a microemulsion (ME) system can replace the conventional oil phase with a medicinal oil and reduce the use of chemical materials. Medium- and short-chain alcohols are typically used as a cosurfactant in conventional ME, but it may cause adverse reactions. It is necessary to prepare alcohol-free ME. One alternative to alcohol-free preparation is the use of linker molecules. In this study, an emodin-loaded, linker-based CSO ME (Em-L-CSO-ME) was prepared. EL-35 was used as the surfactant, Span-80 was used as the lipophilic linker, acetic acid/sodium acetate was used as the hydrophilic linker, CSO was used as the oil phase and water was used as the aqueous phase. The droplet size of Em-L-CSO-ME was 37.96 ± 0.52 nm, the PDI was 0.13 ± 0.04, the Zeta potential was −0.94 ± 0.20 mV, and the entrapment efficiency was 93.36 ± 0.83%. Analysis of the rate of cancer cell inhibition using A549 cells showed that Em-L-CSO-ME inhibited the proliferation of A549 cells in a dose-dependent manner. Additionally, Em-L-CSO-ME worked just as effectively as ME prepared with conventional cosurfactants. Then, particle dissipation dynamics (DPD) was used to study the phase behavior of the linker-based CSO ME (L-CSO-ME). The results showed that the phase behavior of L-CSO-ME may be related to the interaction between phases, which was enhanced by linker molecules and increased the solubility of the CSO. DPD directly reflected the phase behavior of the linker molecule that promoted the formation of the ME, which is difficult to prove experimentally. The results showed that the linker could provide an alternative preparation strategy for CSO ME.

ix仁油（CSO）是从co仁中提取的一种脂肪油，具有广泛的抗癌作用。在微乳液（ME）系统中使用CSO作为油相可以用药用油代替常规油相，并减少化学材料的使用。在常规ME中，中链和短链醇通常用作助表面活性剂，但可能引起不良反应。有必要准备不含酒精的ME。无酒精制备的一种替代方法是使用连接分子。在这项研究中，制备了基于大黄素的，基于连接子的CSO ME（Em-L-CSO-ME）。EL-35用作表面活性剂，Span-80用作亲脂性连接剂，乙酸/乙酸钠用作亲水性连接剂，CSO用作油相，水用作水相。Em-L-CSO-ME的液滴尺寸为37.96±0.52 nm，PDI为0.13±0.04，Zeta电位为-0.94±0.20 mV，捕获效率为93.36±0.83％。使用A549细胞对癌细胞抑制率的分析表明，Em-L-CSO-ME以剂量依赖性方式抑制A549细胞的增殖。此外，Em-L-CSO-ME的效果与使用常规助表面活性剂制备的ME一样有效。然后，使用粒子耗散动力学（DPD）研究了基于连接子的CSO ME（L-CSO-ME）的相态。结果表明，L-CSO-ME的相行为可能与相之间的相互作用有关，连接分子增强了C-SO的溶解度。DPD直接反映了促进ME形成的接头分子的相行为，这在实验上难以证明。